Analytical Data
-
Gene name
ANKK1
- Application
-
Alternative Names
ANKK1; PKK2; SGK288Ankyrin repeat and Protein kinase domain-containing Protein 1
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q8NFD2
-
Expression Region
1-765aa
-
AA Sequence
MAADPTELRLGSLPVFTRDDFEGDWRLVASGGFSQVFQARHRRWRTEYAIKCAPCLPPDAASSDVNYLIEEAAKMKKIKFQHIVSIYGVCKQPLGIVMEFMANGSLEKVLSTHSLCWKLRFRIIHETSLAMNFLHSIKPPLLHLDLKPGNILLDSNMHVKISDFGLSKWMEQSTRMQYIERSALRGMLSYIPPEMFLESNKAPGPKYDVYSFAIVIWELLTQKKPYSGFNMMMIIIRVAAGMRPSLQPVSDQWPSEAQQMVDLMKRCWDQDPKKRPCFLDITIETDILLSLLQSRVAVPESKALARKVSCKLSLRQPGEVNEDISQELMDSDSGNYLKRALQLSDRKNLVPRDEELCIYENKVTPLHFLVAQGSVEQVRLLLAHEVDVDCQTASGYTPLLIAAQDQQPDLCALLLAHGADANRVDEDGWAPLHFAAQNGDDGTARLLLDHGACVDAQEREGWTPLHLAAQNNFENVARLLVSRQADPNLHEAEGKTPLHVAAYFGHVSLVKLLTSQGAELDAQQRNLRTPLHLAVERGKVRAIQHLLKSGAVPDALDQSGYGPLHTAAARGKYLICKMLLRYGASLELPTHQGWTPLHLAAYKGHLEIIHLLAESHANMGALGAVNWTPLHLAARHGEEAVVSALLQCGADPNAAEQSGWTPLHLAVQRSTFLSVINLLEHHANVHARNKVGWTPAHLAALKGNTAILKVLVEAGAQLDVQDGVSCTPLQLALRSRKQGIMSFLEGKEPSVATLGGSKPGAEMEI
-
Molecular Weight
84.2 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ANKK1 (ankyrin repeat and kinase domain containing 1) is a gene that encodes a protein involved in various cellular processes, including signal transduction and neuronal function. Research has found ANKK1 to be associated with various psychological and neurodegenerative disorders, including Parkinson's disease and addiction. The relevance of ANKK1 in dopamine signaling pathways, particularly those involving D2 dopamine receptors, has garnered interest due to its potential implications for understanding the neurobiological underpinnings of these conditions. Given its pivotal role, the expression and functional characterization of recombinant ANKK1 protein have become a focal point in neurobiological research. By producing ANKK1 as a recombinant protein, scientists aim to elucidate its structural properties, enzymatic activity, and interactions with other proteins. This approach not only aids in understanding the molecular mechanisms of disease but also has therapeutic potential. The study of ANKK1 reconstituted in vitro allows for controlled experimental conditions, shedding light on its role in cell signaling pathways. Furthermore, understanding the structure-function relationship of this protein could lead to the development of targeted therapies, particularly for disorders influenced by dopamine dysregulation. Thus, ongoing research on ANKK1 recombinant protein represents a crucial avenue for unlocking new insights into complex neurological and psychiatric disorders.
