Analytical Data
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Gene name
AMID
- Application
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Alternative Names
ybjR
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P75820
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Expression Region
17-276aa
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AA Sequence
CAGEKGIVEKEGYQLDTRRQAQAAYPRIKVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGISAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKPENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASLLELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
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Molecular Weight
34.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AMID (apoptosis-inducing factor, mitochondria-associated 1) is a critical protein involved in various cellular processes, including apoptosis and mitochondrial function. As a member of the AIF family, AMID plays a pivotal role in mediating cell death responses under stress conditions, highlighting its importance in cancer biology and neurodegenerative diseases. Understanding the molecular mechanisms governing AMID's role in apoptosis can provide insights into potential therapeutic targets for these conditions. The study of recombinant AMID protein allows researchers to investigate its structural and functional properties in detail. By expressing AMID in various systems, scientists can produce large quantities of the protein for biochemical assays, structural analysis, and functional studies. Furthermore, elucidating the interaction of AMID with other cellular components can shed light on its regulatory pathways and contribute to our knowledge of cell survival and death mechanisms. The research into AMID's recombinant form is crucial for developing novel strategies to manipulate apoptotic pathways, ultimately aiding in the discovery of targeted therapies for diseases characterized by dysregulated cell death. Thus, AMID's recombinant protein research serves as a foundation for understanding its role in cellular homeostasis and the potential implications for treating various diseases.
