Analytical Data
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基因名
TAF10
- Application
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别名
TAF10;TAF2A;Transcription initiation factor TFIID subunit 10
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q12962
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表达区间
84-218aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSGAAPPEG AISNGVYVLP SAANGDVKPV VSSTPLVDFL MQLEDYTPTI PDAVTGYYLN RAGFEASDPR IIRLISLAAQ KFISDIANDA LQHCKMKGTA SGSSRSKSKD RKYTLTMEDL TPALSEYGIN VKKPHYFT
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分子量
17 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TAF10, a core component of the TFIID complex, plays a crucial role in transcriptional regulation and gene expression. As part of the multiprotein complex, TAF10 interacts with other general transcription factors and RNA polymerase II, facilitating the recruitment of these essential components to promoter regions of DNA. Understanding TAF10's structure and function has notable implications in both basic and applied molecular biology. Recent research has highlighted its involvement in various cellular processes, including cell growth, differentiation, and response to stress, linking it to essential pathways that govern cellular homeostasis. Mutations and dysregulation of TAF10 have been implicated in several human diseases, including cancer, making it a potential biomarker and therapeutic target. The recombinant protein expression of TAF10 allows for in-depth biochemical and biophysical studies, which can elucidate its functional mechanisms and interactions within the TFIID complex. Moreover, the production of TAF10 as a recombinant protein enables researchers to explore its interactions with various transcription factors and small molecule inhibitors, paving the way for innovative approaches to modulate gene expression for therapeutic purposes. Overall, the research on TAF10 recombinant protein is pivotal in uncovering its multifaceted roles within the cell and developing strategies to address diseases linked to transcriptional dysregulation.












