Analytical Data
-
基因名
XPA
- Application
-
别名
XPA；XPAC；DNA repair Protein complementing XP-A cells
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P23025
-
表达区间
1-273aa
-
氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSMAAADGA LPEAAALEQP AELPASVRAS IERKRQRALM LRQARLAARP YSATAAAATG GMANVKAAPK IIDTGGGFIL EEEEEEEQKI GKVVHQPGPV MEFDYVICEE CGKEFMDSYL MNHFDLPTCD NCRDADDKHK LITKTEAKQE YLLKDCDLEK REPPLKFIVK KNPHHSQWGD MKLYLKLQIV KRSLEVWGSQ EALEEAKEVR QENREKMKQK KFDKKVKELR RAVRSSVWKR ETIVHQHEYG PEENLEDDMY RKTCTMCGHE LTYEKM
-
分子量
34 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The XPA protein, a crucial component of the nucleotide excision repair (NER) pathway, plays a significant role in the cellular response to DNA damage, particularly lesions caused by ultraviolet (UV) radiation and various chemical agents. In humans, mutations in the XPA gene are linked to Xeroderma Pigmentosum (XP), a genetic disorder characterized by extreme sensitivity to UV light, leading to a high predisposition for skin cancers. Research on recombinant XPA protein has gained momentum to understand its structural and functional properties, which are essential for the NER process. The ability to produce recombinant XPA allows for in-depth studies regarding its interactions with other repair proteins and its role in recognizing DNA damage. Moreover, understanding the protein's mechanism can contribute to the development of potential therapeutic strategies for XP patients. The study of recombinant XPA also facilitates the investigation of polymorphisms and their impact on individual susceptibility to environmental carcinogens. Overall, the exploration of XPA recombinants is pivotal for unraveling the complexities of DNA repair mechanisms and addressing the implications of their dysfunction in human health.
