Analytical Data
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Gene name
ZP1
- Application
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Alternative Names
ZP1；Zona pellucida sperm-binding Protein 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P60852
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Expression Region
26-601aa
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AA Sequence
RWLQPDPGLPGLRHSYDCGIKGMQLLVFPRPGQTLRFKVVDEFGNRFDVNNCSICYHWVT SRPQEPAVFSADYRGCHVLEKDGRFHLRVFMEAVLPNGRVDVAQDATLICPKPDPSRTLD SQLAPPAMFSVSTPQTLSFLPTSGHTSQGSGHAFPSPLDPGHSSVHPTPALPSPGPGPTL ATLAQPHWGTLEHWDVNKRDYIGTHLSQEQCQVASGHLPCIVRRTSKEACQQAGCCYDNT REVPCYYGNTATVQCFRDGYFVLVVSQEMALTHRITLANIHLAYAPTSCSPTQHTEAFVV FYFPLTHCGTTMQVAGDQLIYENWLVSGIHIQKGPQGSITRDSTFQLHVRCVFNASDFLP IQASIFPPPSPAPMTQPGPLRLELRIAKDETFSSYYGEDDYPIVRLLREPVHVEVRLLQR TDPNLVLLLHQCWGAPSANPFQQPQWPILSDGCPFKGDSYRTQMVALDGATPFQSHYQRF TVATFALLDSGSQRALRGLVYLFCSTSACHTSGLETCSTACSTGTTRQRRSSGHRNDTAR PQDIVSSPGPVGFEDSYGQEPTLGPTDSNGNSSLRP
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Molecular Weight
70 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ZP1 recombinant protein is a product of increasing interest in the field of reproductive biology and immunology. ZP1 is one of the key components of the zona pellucida, a glycoprotein layer surrounding oocytes, which plays a crucial role in fertilization and early embryonic development. Understanding the dynamics of ZP1 and its interactions with sperm is essential for advancing reproductive technologies and addressing infertility issues. Research on ZP1 recombinant protein aims to elucidate its role in sperm-egg recognition and binding, which is fundamental for successful fertilization. Moreover, ZP1's immunogenic properties can be explored for vaccine development against certain reproductive tract infections. Scientists are conducting various studies to maintain and enhance the biological activity of ZP1 when expressed as a recombinant protein, as this can potentially lead to therapeutic applications in reproductive health and fertility preservation strategies. Furthermore, recombinant ZP1 can also serve as an essential tool for studying the molecular mechanisms of fertilization and the development of assisted reproductive technologies, such as in vitro fertilization (IVF). As research progresses, ZP1 is increasingly recognized not only for its pivotal role in reproduction but also for its broader implications in fertility research and potential biotechnological applications. The exploration of ZP1 recombinant protein is expected to provide significant insights into reproductive biology, enhancing our understanding of gamete interaction and informing future interventions or therapies aimed at overcoming infertility challenges.
