Analytical Data
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基因名
ALKBH8
- Application
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别名
8030431D03Rik; 9430088N01Rik; ABH8; alkB alkylation repair homolog 8 (E. coli)
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96BT7
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表达区间
1-238aa
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氨基酸序列
MDSNHQSNYKLSKTEKKFLRKQIKAKHTLLRHEGIETVSYATQSLVVANGGLGNGVSRNQLLPVLEKCGLVDALLMPPNKPYSFARYRTTEESKRAYVTLNGKEVVDDLGQKITLYLNFVEKVQWKELRPQALPPGLMVVEEIISSEEEKMLLESVDWTEDTDNQNSQKSLKHRRVKHFGYEFHYENNNVDKDKPLSGGLPDICESFLEKWLRKGYIKHKPDQMTINQYEPGQDCHGF
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分子量
53.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ALKBH8 (AlkB homolog 8) is a member of the AlkB family of proteins, which are known for their role in the repair of oxidative damage to RNA. This family includes proteins that utilize Fe(II) and α-ketoglutarate to demethylate various RNA methyl marks, thereby playing a crucial role in RNA metabolism and cellular response to stress. Research on ALKBH8 has gained significance due to its potential involvement in various biological processes, including RNA stability, gene expression regulation, and possibly even cancer progression. Given that RNA methylation can affect the translation and stability of RNA, understanding ALKBH8's function and mechanism could reveal novel insights into the post-transcriptional regulation of gene expression. Moreover, aberrations in ALKBH proteins have been linked to several diseases, underscoring the importance of elucidating the structure and function of ALKBH8. Current research focuses on producing recombinant ALKBH8 proteins to study their enzymatic activities, protein interactions, and structural characteristics, which may inform our understanding of their biological significance and therapeutic potential. As a growing field of interest, this research may pave the way for new insights into RNA biology and its implications in health and disease.
