Analytical Data
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Gene name
AHI1
- Application
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Alternative Names
AHI 1; AHI-1; Ahi1; AHI1_HUMAN; Contatins SH3 and WD40 domains
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8N157
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Expression Region
1-609aa
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AA Sequence
MPTAESEAKVKTKVRFEELLKTHSDLMREKKKLKKKLVRSEENISPDTIRSNLHYMKETTSDDPDTIRSNLPHIKETTSDDVSAANTNNLKKSTRVTKNKLRNTQLATENPNGDASVEEDKQGKPNKKVIKTVPQLTTQDLKPETPENKVDSTHQKTHTKPQPGVDHQKSEKANEGREETDLEEDEELMQAYQCHVTEEMAKEIKRKIRKKLKEQLTYFPSDTLFHDDKLSSEKRKKKKEVPVFSKAETSTLTISGDTVEGEQKKESSVRSVSSDSHQDDEISSMEQSTEDSMQDDTKPKPKKTKKKTKAVADNNEDVDGDGVHEITSRDSPVYPKCLLDDDLVLGVYIHRTDRLKSDFMISHPMVKIHVVDEHTGQYVKKDDSGRPVSSYYEKENVDYILPIMTQPYDFKQLKSRLPEWEEQIVFNENFPYLLRGSDESPKVILFFEILDFLSVDEIKNNSEVQNQECGFRKIAWAFLKLLGANGNANINSKLRLQLYYPPTKPRSPLSVVEAFEWWSKCPRNHYPSTLYVTVRGLKVPDCIKPSYRSMMALQEEKGKPVHCERHHESSSVDTEPGLEESKEVIKWKRLPGQENEDVFVLISPTMEEY
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Molecular Weight
96.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
AHI1 (Abelson Helper Integration site 1) is a protein that has garnered significant attention in biomedical research due to its role in various cellular processes and its association with genetic disorders. Originally identified as a factor that facilitates the integration of the Abelson murine leukemia virus into the host genome, AHI1 has been implicated in the development of the central nervous system. Mutations in the AHI1 gene are linked to Joubert syndrome, a neurodevelopmental disorder characterized by cerebellar and brainstem malformations. The study of AHI1 recombinant protein is crucial for understanding its structure-function relationships and interactions with other proteins, which may shed light on the molecular mechanisms underlying the pathogenesis of related diseases. Researchers utilize recombinant protein techniques to express and purify AHI1, allowing for detailed biochemical assays and structural analyses. Insights gained from these studies can lead to the identification of potential therapeutic targets and strategies for diseases associated with AHI1 dysfunction. Overall, the research on AHI1 recombinant protein not only contributes to our understanding of its biological functions but also opens avenues for translational medicine in neurodevelopmental disorders.
