Analytical Data
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基因名
LIAS
- Application
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别名
LIAS；LAS；Lipoyl synthase. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O43766
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表达区间
28-372aa
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氨基酸序列
LSSLPDKKKELLQNGPDLQDFVSGDLADRSTWDEYKGNLKRQKGERLRLPPWLKTEIPMGKNYNKLKNTLRNLNLHTVCEEARCPNIGECWGGGEYATATATIMLMGDTCTRGCRFCSVKTARNPPPLDASEPYNTAKAIAEWGLDYVVLTSVDRDDMPDGGAEHIAKTVSYLKERNPKILVECLTPDFRGDLKAIEKVALSGLDVYAHNVETVPELQSKVRDPRANFDQSLRVLKHAKKVQPDVISKTSIMLGLGENDEQVYATMKALREADVDCLTLGQYMQPTRRHLKVEEYITPEKFKYWEKVGNELGFHYTASGPLVRSSYKAGEFFLKNLVAKRKTKDL
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分子量
45.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
LIAS, or Lipoic Acid Synthetase, is an important enzyme involved in the biosynthesis of lipoic acid, a vital cofactor required for various metabolic processes, particularly in the mitochondrial multienzyme complexes. The understanding of LIAS has garnered significant attention in recent years due to its crucial role in energy metabolism and its implications in health and disease. Dysfunctions in the LIAS enzyme have been linked to neuromuscular disorders and metabolic syndromes, highlighting the need for a detailed study of its structure and function. Research on recombinant LIAS proteins has become a focal point for elucidating the enzyme's mechanisms and exploring potential therapeutic targets. By studying the properties of LIAS through recombinant techniques, researchers aim to characterize its enzymatic activity, interaction with other proteins, and regulatory pathways. Additionally, this research holds promise for the development of novel strategies to mitigate lipoic acid-related deficiencies and their associated disorders. As the biotechnology field progresses, the recombinant expression of LIAS in various model organisms and systems provides a platform for further understanding its biological significance and potential applications in medicine. This research not only enriches our knowledge of metabolic pathways but also opens new avenues for biotechnological innovations, making LIAS an essential focus in the study of bioenergetics and related fields.
