Analytical Data
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Gene name
AGPAT7
- Application
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Alternative Names
LPCAT4; AGPAT7; AYTL3; LPEAT2; Lysophospholipid acyltransferase LPCAT4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q643R3
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Expression Region
1-524aa
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AA Sequence
MSQGSPGDWAPLDPTPGPPASPNPFVHELHLSRLQRVKFCLLGALLAPIRVLLAFIVLFL LWPFAWLQVAGLSEEQLQEPITGWRKTVCHNGVLGLSRLLFFLLGFLRIRVRGQRASRLQ APVLVAAPHSTFFDPIVLLPCDLPKVVSRAENLSVPVIGALLRFNQAILVSRHDPASRRR VVEEVRRRATSGGKWPQVLFFPEGTCSNKKALLKFKPGAFIAGVPVQPVLIRYPNSLDTT SWAWRGPGVLKVLWLTASQPCSIVDVEFLPVYHPSPEESRDPTLYANNVQRVMAQALGIP ATECEFVGSLPVIVVGRLKVALEPQLWELGKVLRKAGLSAGYVDAGAEPGRSRMISQEEF ARQLQLSDPQTVAGAFGYFQQDTKGLVDFRDVALALAALDGGRSLEELTRLAFELFAEEQ AEGPNRLLYKDGFSTILHLLLGSPHPAATALHAELCQAGSSQGLSLCQFQNFSLHDPLYG KLFSTYLRPPHTSRGTSQTPNASSPGNPTALANGTVQAPKQKGD
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Molecular Weight
83.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AGPAT7 (1-Acylglycerol-3-phosphate O-acyltransferase 7) is a member of the acylglycerol phosphate acyltransferase family, which plays a significant role in lipid metabolism. This enzyme is involved in the regulation of glycerolipid synthesis, contributing to the generation of phosphatidic acid, a key intermediate in lipid biosynthesis and signaling pathways. Research into AGPAT7 has gained momentum due to its potential implications in various metabolic disorders, including obesity, diabetes, and cardiovascular diseases. Alterations in AGPAT7 expression and activity have been linked to insulin resistance and lipid accumulation in adipose tissues. By investigating the structure and function of recombinant AGPAT7, researchers aim to elucidate its biochemical mechanisms, understand its role in cellular lipid homeostasis, and identify potential therapeutic targets for metabolic diseases. Additionally, AGPAT7's involvement in cellular processes such as inflammation and cell proliferation underlines its importance beyond traditional lipid biology, making it a promising candidate for future studies in both basic and translational research. Understanding AGPAT7's functional properties through recombinant technology will aid in the development of novel strategies for combating metabolic disorders and contribute to advancing the field of lipid research.
