Cat: PA2000-5365

Recombinant Human ADAT1 Protein,His

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Analytical Data

  • Gene name

    ADAT1

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    ADAT 1; ADAT1; ADAT1_HUMAN; Adenosine deaminase acting on tRNA

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q9BUB4

  • Expression Region

    1-502aa

  • AA Sequence

    MWTADEIAQL CYEHYGIRLP KKGKPEPNHE WTLLAAVVKI QSPADKACDT PDKPVQVTKE VVSMGTGTKC IGQSKMRKNG DILNDSHAEV IARRSFQRYL LHQLQLAATL KEDSIFVPGT QKGVWKLRRD LIFVFFSSHT PCGDASIIPM LEFEDQPCCP VFRNWAHNSS VEASSNLEAP GNERKCEDPD SPVTKKMRLE PGTAAREVTN GAAHHQSFGK QKSGPISPGI HSCDLTVEGL ATVTRIAPGS AKVIDVYRTG AKCVPGEAGD SGKPGAAFHQ VGLLRVKPGR GDRTRSMSCS DKMARWNVLG CQGALLMHLL EEPIYLSAVV IGKCPYSQEA MQRALIGRCQ NVSALPKGFG VQELKILQSD LLFEQSRSAV QAKRADSPGR LVPCGAAISW SAVPEQPLDV TANGFPQGTT KKTIGSLQAR SQISKVELFR SFQKLLSRIA RDKWPHSLRV QKLDTYQEYK EAASSYQEAW STLRKQVFGS WIRNPPDYHQ FK

  • Molecular Weight

    55.3 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

ADAT1, or tRNA adenosine deaminase 1, is a crucial enzyme involved in the modification of tRNA, responsible for converting adenosine at position 37 to inosine, which plays a significant role in the accuracy and efficiency of protein translation. The study of ADAT1 has gained attention due to its implications in various biological processes and diseases. Abnormalities in tRNA modifications, including those mediated by ADAT1, have been linked to several disorders, including neurological conditions and certain cancers. The recombinant production of ADAT1 offers a valuable tool for investigating its enzymatic properties, structural characteristics, and functional roles in cellular metabolism. By utilizing recombinant DNA technology, researchers can produce large quantities of ADAT1, enabling detailed biophysical and biochemical studies. Understanding ADAT1's mechanisms and potential as a therapeutic target may provide insights into novel strategies for treating diseases associated with tRNA modification defects. Ultimately, the ongoing research on ADAT1 and its recombinant protein could pave the way for advancements in molecular medicine and the development of new biomolecular tools.

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