Analytical Data
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Gene name
ADAT1
- Application
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Alternative Names
ADAT 1; ADAT1; ADAT1_HUMAN; Adenosine deaminase acting on tRNA
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BUB4
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Expression Region
1-502aa
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AA Sequence
MWTADEIAQL CYEHYGIRLP KKGKPEPNHE WTLLAAVVKI QSPADKACDT PDKPVQVTKE VVSMGTGTKC IGQSKMRKNG DILNDSHAEV IARRSFQRYL LHQLQLAATL KEDSIFVPGT QKGVWKLRRD LIFVFFSSHT PCGDASIIPM LEFEDQPCCP VFRNWAHNSS VEASSNLEAP GNERKCEDPD SPVTKKMRLE PGTAAREVTN GAAHHQSFGK QKSGPISPGI HSCDLTVEGL ATVTRIAPGS AKVIDVYRTG AKCVPGEAGD SGKPGAAFHQ VGLLRVKPGR GDRTRSMSCS DKMARWNVLG CQGALLMHLL EEPIYLSAVV IGKCPYSQEA MQRALIGRCQ NVSALPKGFG VQELKILQSD LLFEQSRSAV QAKRADSPGR LVPCGAAISW SAVPEQPLDV TANGFPQGTT KKTIGSLQAR SQISKVELFR SFQKLLSRIA RDKWPHSLRV QKLDTYQEYK EAASSYQEAW STLRKQVFGS WIRNPPDYHQ FK
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Molecular Weight
55.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ADAT1, or tRNA adenosine deaminase 1, is a crucial enzyme involved in the modification of tRNA, responsible for converting adenosine at position 37 to inosine, which plays a significant role in the accuracy and efficiency of protein translation. The study of ADAT1 has gained attention due to its implications in various biological processes and diseases. Abnormalities in tRNA modifications, including those mediated by ADAT1, have been linked to several disorders, including neurological conditions and certain cancers. The recombinant production of ADAT1 offers a valuable tool for investigating its enzymatic properties, structural characteristics, and functional roles in cellular metabolism. By utilizing recombinant DNA technology, researchers can produce large quantities of ADAT1, enabling detailed biophysical and biochemical studies. Understanding ADAT1's mechanisms and potential as a therapeutic target may provide insights into novel strategies for treating diseases associated with tRNA modification defects. Ultimately, the ongoing research on ADAT1 and its recombinant protein could pave the way for advancements in molecular medicine and the development of new biomolecular tools.











