Cat: PA1000-2995

Recombinant Human SOX2 Protein,His

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Analytical Data

  • Gene name

    SOX2

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    SOX2;Transcription factor SOX-2

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    P48431

  • Expression Region

    1-317aa

  • AA Sequence

    MYNMMETELK PPGPQQTSGG GGGNSTAAAA GGNQKNSPDR VKRPMNAFMV WSRGQRRKMA QENPKMHNSE ISKRLGAEWK LLSETEKRPF IDEAKRLRAL HMKEHPDYKY RPRRKTKTLM KKDKYTLPGG LLAPGGNSMA SGVGVGAGLG AGVNQRMDSY AHMNGWSNGS YSMMQDQLGY PQHPGLNAHG AAQMQPMHRY DVSALQYNSM TSSQTYMNGS PTYSMSYSQQ GTPGMALGSM GSVVKSEASS SPPVVTSSSH SRAPCQAGDL RDMISMYLPG AEVPEPAAPS RLHMSQHYQS GPVPGTAING TLPLSHM

  • Molecular Weight

    40.4 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

SOX2, a member of the Sox family of transcription factors, plays a critical role in maintaining pluripotency and self-renewal in embryonic stem cells. Its expression is also pivotal in the development and maintenance of various tissues in adults, including the nervous system. Aberrant regulation of SOX2 has been implicated in various cancers, making it a significant target for therapeutic intervention. Researchers study SOX2 recombinant proteins to elucidate its functional mechanisms and interactions at the molecular level. This involves producing SOX2 proteins through recombinant DNA technology, allowing for detailed investigations into its DNA-binding properties and downstream effects on gene expression. By understanding how SOX2 regulates essential biological processes, researchers aim to unveil its potential as a biomarker for cancer and a target for regenerative medicine. Given its vital roles, SOX2 continues to be a focus of intense study, with the recombinant proteins serving as crucial tools for developing novel diagnostic and therapeutic strategies in regenerative biology and oncology.

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