Analytical Data
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Gene name
NUDT12
- Application
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Alternative Names
NAD-capped RNA hydrolase NUDT12. DeNADding enzyme NUDT12. EC:3.6.1.-. NADH pyrophosphatase NUDT12. EC:3.6.1.22. Nucleoside diphosphate-linked moiety X motif 12. Nudix motif 12
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BQG2
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Expression Region
1-462 aa
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AA Sequence
MSSVKRSLKQEIVTQFHCSAAEGDIAKLTGILSHSPSLLNETSENGWTALMYAARNGHPEIVQFLLEKGCDRSIVNKSRQTALDIAVFWGYKHIANLLATAKGGKKPWFLTNEVEECENYFSKTLLDRKSEKRNNSDWLLAKESHPATVFILFSDLNPLVTLGGNKESFQQPEVRLCQLNYTDIKDYLAQPEKITLIFLGVELEIKDKLLNYAGEVPREEEDGLVAWFALGIDPIAAEEFKQRHENCYFLHPPMPALLQLKEKEAGVVAQARSVLAWHSRYKFCPTCGNATKIEEGGYKRLCLKEDCPSLNGVHNTSYPRVDPVVIMQVIHPDGTKCLLGRQKRFPPGMFTCLAGFIEPGETIEDAVRREVEEESGVKVGHVQYVACQPWPMPSSLMIGCLALAVSTEIKVDKNEIEDARWFTREQVLDVLTKGKQQAFFVPPSRAIAHQLIKHWIRINPNL
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Molecular Weight
76.56 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NUDT12 is a member of the nudix hydrolase family, a group of enzymes that play crucial roles in nucleotide metabolism and cellular signaling by hydrolyzing a variety of nucleotide derivatives. Research on NUDT12 has gained attention due to its potential involvement in various biological processes and diseases, particularly in the context of cellular response to oxidative stress and regulation of nucleotide pools. Preliminary studies have suggested that NUDT12 may play a key role in detoxifying harmful nucleotides, such as nucleotide triphosphates, which can accumulate and cause cellular damage when produced in excess. Additionally, the enzyme’s activity could influence pathways related to inflammation and cancer by modulating intracellular signaling molecules. Understanding the structure and function of NUDT12 through recombinant protein studies can provide insights into its catalytic mechanisms, substrate specificity, and potential regulatory roles in cellular physiology. Furthermore, characterizing NUDT12 may help reveal its potential as a therapeutic target for diseases where nucleotide imbalance and oxidative stress are implicated. Therefore, the exploration of NUDT12 as a recombinant protein not only contributes to fundamental biochemistry but also holds promise for developing novel strategies in disease management.
