Analytical Data
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Gene name
ABHD12
- Application
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Alternative Names
2-arachidonoylglycerol hydrolase; 6330583M11Rik; ABD12_HUMAN; ABHD12
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8N2K0
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Expression Region
1-398aa
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AA Sequence
MRKRTEPVALEHERCAAAGSSSSGSAAAALDADCRLKQNLRLTGPAAAEPRCAADAGMKRALGRRKGVWLRLRKILFCVLGLYIAIPFLIKLCPGIQAKLIFLNFVRVPYFIDLKKPQDQGLNHTCNYYLQPEEDVTIGVWHTVPAVWWKNAQGKDQMWYEDALASSHPIILYLHGNAGTRGGDHRVELYKVLSSLGYHVVTFDYRGWGDSVGTPSERGMTYDALHVFDWIKARSGDNPVYIWGHSLGTGVATNLVRRLCERETPPDALILESPFTNIREEAKSHPFSVIYRYFPGFDWFFLDPITSSGIKFANDENVKHISCPLLILHAEDDPVVPFQLGRKLYSIAAPARSFRDFKVQFVPFHSDLGYRHKYIYKSPELPRILREFLGKSEPEHQH
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Molecular Weight
45.0 KDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ABHD12 (Abhydrolase Domain Containing 12) is a member of the serine hydrolase family, playing a crucial role in lipid metabolism and signaling pathways. Mutations in the ABHD12 gene are associated with the rare neurodegenerative disorder known as Chanarin-Dorfman syndrome, which is characterized by lipid accumulation in various tissues and neurological deficits. The study of recombinant ABHD12 protein has gained significance as it allows researchers to delve into its enzymatic activities, substrate specificity, and the molecular mechanisms underlying its function in cellular processes. Understanding the enzymatic properties of ABHD12 and how it interacts with various lipid substrates can provide insights into its role in maintaining lipid homeostasis and the pathogenesis of associated disorders. Furthermore, recombinant ABHD12 can be employed in drug discovery and development, especially in targeting metabolic pathways altered in diseases. Overall, research on recombinant ABHD12 not only enhances our understanding of its biological significance but also contributes to the potential development of therapeutic strategies for conditions linked to its dysfunction.
