Analytical Data
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基因名
SHC1
- Application
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别名
SHC1;SHC;SHCA;SHC-transforming Protein 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P29353
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表达区间
1-474aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHFNTRTQVTREAISLVCEAVPGAKGATRRRK PCSRPLSSILGRSNLKFAGMPITLTVSTSSLNLMAADCKQIIANHHMQSI SFASGGDPDTMNKLSGGGGRRTRVEGGQLGGEEWTRHGSFVNKPTRGWLH PNDKVMGPGVSYLVRYMGCVEVLQSMRALDAEYVAYVAKDPVNQRACHIL ECPEGLAQDVISTIGQAFELRFKQYLRNPPKLVTPHDRMAGFDGSAWDEE EEEPPDHQYYNDFPGKEPPLGGVVDMRLREGAAPGAARPTAPNAQTPSHL GATLPVGQPVGGDPEVRKQMPPPPPCPAGRELFDDPSYVNVQNLDKARQA VGGAGPPNPAINGSAPRDLFDMKPFEDALRVPPPPQSVSMAEQLRGEPWF HGKLSRREAEALLQLNGDFLVRESTTTPGQYVLTGLQSGQPKHLLLVDPE GVVRTKDHRFESVSHLISYHMDNHLPIISAGSELCLQQPVERKL
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分子量
54 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SHC1, a crucial player in cellular signaling, is a member of the SHC family of proteins that serve as adapters for various growth factor receptors and intracellular signaling pathways. Its role in mediating cell proliferation, differentiation, and survival has made it a subject of intense scientific research, particularly in the context of cancer and metabolic diseases. The SHC1 protein contains specific domains, such as the Src homology 2 (SH2) domain and phosphotyrosine-binding (PTB) domain, which facilitate interactions with phosphorylated proteins, thereby activating downstream signaling cascades like the Ras-MAPK pathway. Dysregulation of SHC1 has been linked to tumorigenesis and poor prognosis in several types of cancer, including breast, colorectal, and prostate cancers. Consequently, understanding the structure, function, and regulatory mechanisms of SHC1 and its interactions with other signaling molecules is vital for developing targeted therapeutic strategies. Recent advances in recombinant protein technology have enabled the production of SHC1 in a form suitable for structural and functional studies, paving the way for enhanced understanding of its role in health and disease. These studies aim to elucidate the molecular mechanisms underlying SHC1 function and its potential as a biomarker or therapeutic target, thus contributing to the broader field of cancer biology and personalized medicine.
