Analytical Data
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Gene name
NADSYN1
- Application
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Alternative Names
Glutamine dependent NAD(+) synthetase; Glutamine-dependent NAD(+) synthetase; NAD(+) synthase [glutamine hydrolyzing]; NAD(+) synthase [glutamine-hydrolyzing]; NAD(+) synthetase 1; NAD(+) synthetase; NADE_HUMAN; nadsyn1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6IA69
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Expression Region
1-706 aa
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AA Sequence
MGRKVTVATC ALNQWALDFE GNLQRILKSI EIAKNRGARY RLGPELEICG YGCWDHYYES DTLLHSFQVL AALVESPVTQ DIICDVGMPV MHRNVRYNCR VIFLNRKILL IRPKMALANE GNYRELRWFT PWSRSRHTEE YFLPRMIQDL TKQETVPFGD AVLVTWDTCI GSEICEELWT PHSPHIDMGL DGVEIITNAS GSHQVLRKAN TRVDLVTMVT SKNGGIYLLA NQKGCDGDRL YYDGCAMIAM NGSVFAQGSQ FSLDDVEVLT ATLDLEDVRS YRAEISSRNL AASRASPYPR VKVDFALSCH EDLLAPISEP IEWKYHSPEE EISLGPACWL WDFLRRSQQA GFLLPLSGGV DSAATACLIY SMCCQVCEAV RSGNEEVLAD VRTIVNQISY TPQDPRDLCG RILTTCYMAS KNSSQETCTR ARELAQQIGS HHISLNIDPA VKAVMGIFSL VTGKSPLFAA HGGSSRENLA LQNVQARIRM VLAYLFAQLS LWSRGVHGGL LVLGSANVDE SLLGYLTKYD CSSADINPIG GISKTDLRAF VQFCIQRFQL PALQSILLAP ATAELEPLAD GQVSQTDEED MGMTYAELSV YGKLRKVAKM GPYSMFCKLL GMWRHICTPR QVADKVKRFF SKYSMNRHKM TTLTPAYHAE NYSPEDNRFD LRPFLYNTSW PWQFRCIENQ VLQLERAEPQ SLDGVD
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Molecular Weight
79.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NADSYN1, or NAD synthetase 1, is an essential enzyme involved in the synthesis of NAD+ (nicotinamide adenine dinucleotide), a crucial cofactor in numerous metabolic processes, including energy metabolism, DNA repair, and cell signaling. Recent research has highlighted the significance of NADSYN1 in maintaining cellular NAD+ levels and its potential involvement in various physiological and pathological conditions. For instance, NADSYN1 has been implicated in neurodegenerative diseases, where disrupted NAD+ homeostasis may contribute to neuronal cell death. Additionally, alterations in NADSYN1 activity have been linked to metabolic disorders, indicating its role in regulating metabolic pathways. Understanding the structure and function of recombinant NADSYN1 protein can provide insights into its catalytic mechanisms and regulatory processes, thereby facilitating the development of therapeutic strategies targeting NAD+ metabolism. The production of recombinant NADSYN1 allows for detailed biochemical studies, enabling researchers to explore its kinetic properties, substrate specificity, and interactions with other biomolecules. Such investigations are essential for elucidating the enzyme's role in health and disease, paving the way for potential interventions that could modulate NAD+ levels to promote cellular health and longevity. Thus, research on recombinant NADSYN1 protein not only expands our fundamental knowledge of NAD+ biosynthesis but also opens new avenues for therapeutic applications in disorders characterized by NAD+ deficiency.
