Analytical Data
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基因名
NADSYN1
- Application
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别名
Glutamine dependent NAD(+) synthetase; Glutamine-dependent NAD(+) synthetase; NAD(+) synthase [glutamine hydrolyzing]; NAD(+) synthase [glutamine-hydrolyzing]; NAD(+) synthetase 1; NAD(+) synthetase; NADE_HUMAN; nadsyn1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q6IA69
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表达区间
1-706 aa
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氨基酸序列
MGRKVTVATC ALNQWALDFE GNLQRILKSI EIAKNRGARY RLGPELEICG YGCWDHYYES DTLLHSFQVL AALVESPVTQ DIICDVGMPV MHRNVRYNCR VIFLNRKILL IRPKMALANE GNYRELRWFT PWSRSRHTEE YFLPRMIQDL TKQETVPFGD AVLVTWDTCI GSEICEELWT PHSPHIDMGL DGVEIITNAS GSHQVLRKAN TRVDLVTMVT SKNGGIYLLA NQKGCDGDRL YYDGCAMIAM NGSVFAQGSQ FSLDDVEVLT ATLDLEDVRS YRAEISSRNL AASRASPYPR VKVDFALSCH EDLLAPISEP IEWKYHSPEE EISLGPACWL WDFLRRSQQA GFLLPLSGGV DSAATACLIY SMCCQVCEAV RSGNEEVLAD VRTIVNQISY TPQDPRDLCG RILTTCYMAS KNSSQETCTR ARELAQQIGS HHISLNIDPA VKAVMGIFSL VTGKSPLFAA HGGSSRENLA LQNVQARIRM VLAYLFAQLS LWSRGVHGGL LVLGSANVDE SLLGYLTKYD CSSADINPIG GISKTDLRAF VQFCIQRFQL PALQSILLAP ATAELEPLAD GQVSQTDEED MGMTYAELSV YGKLRKVAKM GPYSMFCKLL GMWRHICTPR QVADKVKRFF SKYSMNRHKM TTLTPAYHAE NYSPEDNRFD LRPFLYNTSW PWQFRCIENQ VLQLERAEPQ SLDGVD
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分子量
79.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NADSYN1, or NAD synthetase 1, is an essential enzyme involved in the synthesis of NAD+ (nicotinamide adenine dinucleotide), a crucial cofactor in numerous metabolic processes, including energy metabolism, DNA repair, and cell signaling. Recent research has highlighted the significance of NADSYN1 in maintaining cellular NAD+ levels and its potential involvement in various physiological and pathological conditions. For instance, NADSYN1 has been implicated in neurodegenerative diseases, where disrupted NAD+ homeostasis may contribute to neuronal cell death. Additionally, alterations in NADSYN1 activity have been linked to metabolic disorders, indicating its role in regulating metabolic pathways. Understanding the structure and function of recombinant NADSYN1 protein can provide insights into its catalytic mechanisms and regulatory processes, thereby facilitating the development of therapeutic strategies targeting NAD+ metabolism. The production of recombinant NADSYN1 allows for detailed biochemical studies, enabling researchers to explore its kinetic properties, substrate specificity, and interactions with other biomolecules. Such investigations are essential for elucidating the enzyme's role in health and disease, paving the way for potential interventions that could modulate NAD+ levels to promote cellular health and longevity. Thus, research on recombinant NADSYN1 protein not only expands our fundamental knowledge of NAD+ biosynthesis but also opens new avenues for therapeutic applications in disorders characterized by NAD+ deficiency.
