Analytical Data
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Gene name
CHKb
- Application
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Alternative Names
CHKb；CHETK；CHKL；Choline/ethanolamine kinase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y259
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Expression Region
1-395aa
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AA Sequence
MAAEATAVAGSGAVGGCLAKDGLQQSKCPDTTPKRRRASSLSRDAERRAYQWCREYLGGAWRRVQPEELRVYPVSGGLSNLLFRCSLPDHLPSVGEEPREVLLRLYGAILQGVDSLVLESVMFAILAERSLGPQLYGVFPEGRLEQYIPSRPLKTQELREPVLSAAIATKMAQFHGMEMPFTKEPHWLFGTMERYLKQIQDLPPTGLPEMNLLEMYSLKDEMGNLRKLLESTPSPVVFCHNDIQEGNILLLSEPENADSLMLVDFEYSSYNYRGFDIGNHFCEWVYDYTHEEWPFYKARPTDYPTQEQQLHFIRHYLAEAKKGETLSQEEQRKLEEDLLVEVSRYALASHFFWGLWSILQASMSTIEFGYLDYAQSRFQFYFQQKGQLTSVHSSS
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Molecular Weight
45.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CHKb (Checkpoint Kinase 2) is a crucial protein involved in the DNA damage response and cell cycle regulation, playing a significant role in maintaining genomic integrity. Its dysregulation is associated with various cancers, making it a key target for therapeutic interventions. Research on CHKb recombinant protein has gained momentum due to its potential applications in cancer therapy and diagnostics. The ability to produce CHKb in a recombinant form allows for detailed biochemical studies, enabling researchers to explore its function, interactions, and regulatory mechanisms. This can provide insights into how CHKb mediates cellular responses to DNA damage and stress, contributing to our understanding of tumorigenesis. Additionally, recombinant CHKb can serve as a valuable tool for developing inhibitors that could selectively target and modulate its activity in cancer cells. Such advancements can lead to novel strategies for cancer treatment, highlighting the importance of CHKb research in the broader context of cancer biology and therapy. By systematically studying CHKb and its pathways, researchers hope to uncover new biomarkers and therapeutic targets that could enhance the efficacy of existing cancer treatments and improve patient outcomes.
