Analytical Data
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基因名
PYCRL
- Application
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别名
PYCR3;PYCRL;Pyrroline-5-carboxylate reductase 3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q53H96
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表达区间
1-274aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSMAAAEPS PRRVGFVGAG RMAGAIAQGL IRAGKVEAQH ILASAPTDRN LCHFQALGCR TTHSNQEVLQ SCLLVIFATK PHVLPAVLAE VAPVVTTEHI LVSVAAGVSL STLEELLPPN TRVLRVLPNL PCVVQEGAIV MARGRHVGSS ETNLLQHLLE ACGRCEEVPE AYVDIHTGLS GSGVAFVCAF SEALAEGAVK MGMPSSLAHR IAAQTLLGTA KMLLHEGQHP AQLRSDVCTP GGTTIYGLHA LEQGGLRAAT MSAVEAATCR AKELSRK
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分子量
31 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PYCRL, or Pyrroline-5-carboxylate reductase-like protein, is a member of the pyrroline-5-carboxylate reductase family, which plays a crucial role in proline metabolism. This protein is believed to be involved in various physiological processes, including cellular stress responses and nitric oxide signaling. The study of PYCRL has gained attention due to its potential implications in several pathological conditions, such as neurodegenerative diseases and cancer, where proline metabolism is often dysregulated. Understanding the structure and function of PYCRL could provide insights into its role in cellular homeostasis and vulnerability to oxidative stress. Furthermore, the recombinant expression of PYCRL allows for detailed biochemical characterization, which can elucidate its enzymatic properties and regulatory mechanisms. These studies are essential for exploring PYCRL as a potential therapeutic target or biomarker in diseases linked to metabolic dysregulation, highlighting the importance of this protein in both basic and applied biomedical research.
