Analytical Data
-
基因名
PYCR1
- Application
-
别名
PYCR1;Pyrroline-5-carboxylate reductase 1. mitochondrial
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P32322
-
表达区间
1-319aa
-
氨基酸序列
MGSSHHHHHHSSGLVPRGSHMSVGFIGAGQLAFALAKGFTAAGVLAAHKI MASSPDMDLATVSALRKMGVKLTPHNKETVQHSDVLFLAVKPHIIPFILD EIGADIEDRHIVVSCAAGVTISSIEKKLSAFRPAPRVIRCMTNTPVVVRE GATVYATGTHAQVEDGRLMEQLLSSVGFCTEVEEDLIDAVTGLSGSGPAY AFTALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLHSEQHPGQLKDN VSSPGGATIHALHVLESGGFRSLLINAVEASCIRTRELQSMADQEQVSPA AIKKTILDKVKLDSPAGTALSPSGHTKLLPRSLAPAGKD
-
分子量
36 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PYCR1, or pyrroline-5-carboxylate reductase 1, is an enzyme that plays a crucial role in the proline biosynthetic pathway, which is essential for maintaining cellular homeostasis, particularly under stress conditions. Research has indicated that PYCR1 is involved in the conversion of pyrroline-5-carboxylate to proline, an amino acid that serves as an important osmoprotectant, antioxidant, and regulator of cellular signaling. Dysregulation of PYCR1 has been linked to various pathological conditions, including metabolic disorders, neurodegenerative diseases, and cancer, highlighting its significance in cellular health and disease. The study of recombinant PYCR1 protein has gained traction as it provides insights into its biochemical properties, functional mechanisms, and potential therapeutic applications. Understanding the structure-function relationship of PYCR1 at a molecular level can unveil targets for drug development and interventions in related diseases. Moreover, recombinant PYCR1 can serve as a valuable tool in metabolic engineering and synthetic biology, allowing for the manipulation of proline metabolism in various organisms. Investigating this protein can therefore contribute to a broader understanding of metabolic pathways and their implications in health and disease management.
