Analytical Data
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Gene name
PYCR2
- Application
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Alternative Names
PYCR2;Pyrroline-5-carboxylate reductase 2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96C36
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Expression Region
2-320aa
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AA Sequence
SVGFIGAGQ LAYALARGFT AAGILSAHKI IASSPEMNLP TVSALRKMGV NLTRSNKETV KHSDVLFLAV KPHIIPFILD EIGADVQARH IVVSCAAGVT ISSVEKKLMA FQPAPKVIRC MTNTPVVVQE GATVYATGTH ALVEDGQLLE QLMSSVGFCT EVEEDLIDAV TGLSGSGPAY AFMALDALAD GGVKMGLPRR LAIQLGAQAL LGAAKMLLDS EQHPCQLKDN VCSPGGATIH ALHFLESGGF RSLLINAVEA SCIRTRELQS MADQEKISPA ALKKTLLDRV KLESPTVSTL TPSSPGKLLT RSLALGGKKD
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PYCR2, or Pyrroline-5-carboxylate reductase 2, is an enzyme involved in proline metabolism, playing a critical role in the regulation of cellular redox states and stress responses. Its function is essential in the conversion of pyrroline-5-carboxylate (P5C) to proline, an amino acid that serves as an important osmoprotectant and growth regulator during conditions of oxidative stress. Elevated levels of proline contribute to cellular protection from environmental stresses, including drought and salinity, making PYCR2 particularly relevant in plant studies aimed at enhancing stress resilience. Furthermore, mutations or dysregulation of PYCR2 have been linked to various pathological conditions, including metabolic disorders and cancer, highlighting its potential as a therapeutic target. Consequently, research into the recombinant form of PYCR2 facilitates the elucidation of its structure and function, allowing scientists to better understand its involvement in metabolic pathways and its broader implications in both plant physiology and human health. Through recombinant techniques, the purification and characterization of PYCR2 can lead to insights into its enzymatic properties, substrate specificity, and regulatory mechanisms, ultimately contributing to the development of strategies for improving crop performance under stress and exploring its therapeutic potentials in medical contexts.
