Analytical Data
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基因名
PYCR2
- Application
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别名
PYCR2;Pyrroline-5-carboxylate reductase 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96C36
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表达区间
2-320aa
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氨基酸序列
SVGFIGAGQ LAYALARGFT AAGILSAHKI IASSPEMNLP TVSALRKMGV NLTRSNKETV KHSDVLFLAV KPHIIPFILD EIGADVQARH IVVSCAAGVT ISSVEKKLMA FQPAPKVIRC MTNTPVVVQE GATVYATGTH ALVEDGQLLE QLMSSVGFCT EVEEDLIDAV TGLSGSGPAY AFMALDALAD GGVKMGLPRR LAIQLGAQAL LGAAKMLLDS EQHPCQLKDN VCSPGGATIH ALHFLESGGF RSLLINAVEA SCIRTRELQS MADQEKISPA ALKKTLLDRV KLESPTVSTL TPSSPGKLLT RSLALGGKKD
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PYCR2, or Pyrroline-5-carboxylate reductase 2, is an enzyme involved in proline metabolism, playing a critical role in the regulation of cellular redox states and stress responses. Its function is essential in the conversion of pyrroline-5-carboxylate (P5C) to proline, an amino acid that serves as an important osmoprotectant and growth regulator during conditions of oxidative stress. Elevated levels of proline contribute to cellular protection from environmental stresses, including drought and salinity, making PYCR2 particularly relevant in plant studies aimed at enhancing stress resilience. Furthermore, mutations or dysregulation of PYCR2 have been linked to various pathological conditions, including metabolic disorders and cancer, highlighting its potential as a therapeutic target. Consequently, research into the recombinant form of PYCR2 facilitates the elucidation of its structure and function, allowing scientists to better understand its involvement in metabolic pathways and its broader implications in both plant physiology and human health. Through recombinant techniques, the purification and characterization of PYCR2 can lead to insights into its enzymatic properties, substrate specificity, and regulatory mechanisms, ultimately contributing to the development of strategies for improving crop performance under stress and exploring its therapeutic potentials in medical contexts.
