Analytical Data
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Gene name
MED8
- Application
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Alternative Names
MED8；Mediator of RNA polymerase II transcription subunit 8
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96G25
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Expression Region
1-268aa
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AA Sequence
MQREEKQLEASLDALLSQVADLKNSLGSFICKLENEYGRLTWPSVLDSFALLSGQLNTLNKVLKHEKTPLFRNQVIIPLVLSPDRDEDLMRQTEGRVPVFSHEVVPDHLRTKPDPEVEEQEKQLTTDAARIGADAAQKQIQSLNKMCSNLLEKISKEERESESGGLRPNKQTFNPTDTNALVAAVAFGKGLSNWRPSGSSGPGQAGQPGAGTILAGTSGLQQVQMAGAPSQQQPMLSGVQMAQAGQPGKMPSGIKTNIKSASMHPYQR
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Molecular Weight
29 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MED8 is a critical subunit of the Mediator complex, which plays an essential role in regulating gene expression by serving as a bridge between transcription factors and RNA polymerase II. Research on MED8 has gained prominence due to its involvement in various biological processes, including development, differentiation, and response to environmental stimuli. Mutations or dysregulation of MED8 have been implicated in several diseases, including cancer, highlighting its potential as a therapeutic target. Recent studies have employed advanced techniques, such as cryo-electron microscopy and X-ray crystallography, to elucidate the structural dynamics of MED8 within the Mediator complex. These insights can reveal how MED8 interacts with other subunits and transcription factors, which is crucial for understanding the molecular mechanisms of gene regulation. Additionally, the characterization of MED8's post-translational modifications has shown how these alterations can influence its function and stability, further emphasizing its importance in the context of cellular responses. As research progresses, MED8 continues to be a focal point for understanding the intricate web of transcriptional control, offering promising avenues for innovative approaches in treating related disorders.
