Analytical Data
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基因名
CHKa
- Application
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别名
CHKa；CHK；CKI；Choline kinase alpha
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P35790
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表达区间
1-457aa
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氨基酸序列
MKTKFCTGGEAEPSPLGLLLSCGSGSAAPAPGVGQQRDAASDLESKQLGG QQPPLALPPPPPLPLPLPLPQPPPPQPPADEQPEPRTRRRAYLWCKEFLP GAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYGA ILQMRSCNKEGSEQAQKENEFQGAEAMVLESVMFAILAERSLGPKLYGIF PQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLF GTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPV VFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEW MYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSI IKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFH QKRKLGV
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分子量
76 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CHKa, or Chlamydia Helicase A, is an essential protein involved in the replication of Chlamydia trachomatis, a significant pathogen responsible for various human infections, including sexually transmitted diseases and blindness. The study of CHKa has gained importance due to its crucial role in the bacterial lifecycle, particularly in DNA unwinding during replication and transcription processes. As antibiotic resistance continues to pose challenges in treating Chlamydia infections, understanding the structure and function of CHKa may provide insights into novel therapeutic targets. Research has demonstrated that CHKa exhibits helicase activity, which is vital for the bacterium's survival and pathogenicity. In recent years, efforts have been focused on characterizing CHKa's biochemical properties and its interactions with other molecular components, aiming to develop inhibitors that can disrupt its function. Additionally, structural studies using techniques like X-ray crystallography and cryo-electron microscopy have begun to elucidate the conformational dynamics of CHKa, paving the way for the development of structure-based drug design strategies. Overall, the investigation of CHKa is not only significant for understanding Chlamydia biology but also holds the potential to advance public health by providing new avenues for treating infections that currently lack effective solutions.
