Analytical Data
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Gene name
CHKa
- Application
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Alternative Names
CHKa；CHK；CKI；Choline kinase alpha
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P35790
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Expression Region
1-457aa
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AA Sequence
MKTKFCTGGEAEPSPLGLLLSCGSGSAAPAPGVGQQRDAASDLESKQLGG QQPPLALPPPPPLPLPLPLPQPPPPQPPADEQPEPRTRRRAYLWCKEFLP GAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYGA ILQMRSCNKEGSEQAQKENEFQGAEAMVLESVMFAILAERSLGPKLYGIF PQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLF GTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPV VFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEW MYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSI IKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFH QKRKLGV
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Molecular Weight
76 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CHKa, or Chlamydia Helicase A, is an essential protein involved in the replication of Chlamydia trachomatis, a significant pathogen responsible for various human infections, including sexually transmitted diseases and blindness. The study of CHKa has gained importance due to its crucial role in the bacterial lifecycle, particularly in DNA unwinding during replication and transcription processes. As antibiotic resistance continues to pose challenges in treating Chlamydia infections, understanding the structure and function of CHKa may provide insights into novel therapeutic targets. Research has demonstrated that CHKa exhibits helicase activity, which is vital for the bacterium's survival and pathogenicity. In recent years, efforts have been focused on characterizing CHKa's biochemical properties and its interactions with other molecular components, aiming to develop inhibitors that can disrupt its function. Additionally, structural studies using techniques like X-ray crystallography and cryo-electron microscopy have begun to elucidate the conformational dynamics of CHKa, paving the way for the development of structure-based drug design strategies. Overall, the investigation of CHKa is not only significant for understanding Chlamydia biology but also holds the potential to advance public health by providing new avenues for treating infections that currently lack effective solutions.
