Analytical Data
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基因名
SEPN1
- Application
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别名
SEPN1；SELN；SEPN1；SelenoProtein N
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NZV5
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表达区间
44-590aa
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氨基酸序列
AAVRVCA RHAEAQAAAR QELALKTLGT DGLFLFSSLD TDGDMYISPE EFKPIAEKLT GSCSVTQTGV QWCSHSSLQP QLPWLNUSSC LSLLRSTPAA SCEEEELPPD PSEETLTIEA RFQPLLPETM TKSKDGFLGV SRLALSGLRN WTAAASPSAV FATRHFQPFL PPPGQELGEP WWIIPSELSM FTGYLSNNRF YPPPPKGKEV IIHRLLSMFH PRPFVKTRFA PQGAVACLTA ISDFYYTVMF RIHAEFQLSE PPDFPFWFSP AQFTGHIILS KDATHVRDFR LFVPNHRSLN VDMEWLYGAS ESSNMEVDIG YIPQMELEAT GPSVPSVILD EDGSMIDSHL PSGEPLQFVF EEIKWQQELS WEEAARRLEV AMYPFKKVSY LPFTEAFDRA KAENKLVHSI LLWGALDDQS CUGSGRTLRE TVLESSPILT LLNESFISTW SLVKELEELQ NNQENSSHQK LAGLHLEKYS FPVEMMICLP NGTVVHHINA NYFLDITSVK PEEIESNLFS FSSTFEDPST ATYMQFLKEG LRRGLPLLQP
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分子量
65.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SEPN1, or selenoprotein N, is a critical gene that encodes a protein involved in various cellular processes, particularly in muscle function and oxidative stress response. Mutations in the SEPN1 gene have been linked to distinct myopathies, such as congenital myopathy and rigid spine syndrome, characterized by muscle weakness and structural defects. The protein product is unique due to its incorporation of the selenium amino acid selenocysteine, which is essential for its function. Research into SEPN1 recombinant protein has gained traction as it provides insights into protein structure, stability, and activity, facilitating the understanding of its role in muscle biology and disease mechanisms. Additionally, the study of SEPN1 can pave the way for potential therapeutic approaches, including the development of gene therapies or targeted treatments aimed at ameliorating the effects of SEPN1-related disorders. Given the importance of selenoproteins in human health, the exploration of SEPN1’s functional properties, interactions with other proteins, and its involvement in cellular processes remains a significant area of interest in biomedical research. By generating and characterizing recombinant SEPN1 proteins, researchers aim to elucidate the molecular pathways influenced by this selenoprotein, ultimately contributing to the development of innovative strategies to manage selenoprotein deficiencies and related muscle disorders.
