Analytical Data
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基因名
PINK1
- Application
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别名
PINK1;Serine/threonine-Protein kinase PINK1. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9BXM7
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表达区间
156-507aa
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氨基酸序列
MYLIGQSIGK GCSAAVYEAT MPTLPQNLEV TKSTGLLPGR GPGTSAPGEG QERAPGAPAF PLAIKMMWNI SAGSSSEAIL NTMSQELVPA SRVALAGEYG AVTYRKSKRG PKQLAPHPNI IRVLRAFTSS VPLLPGALVD YPDVLPSRLH PEGLGHGRTL FLVMKNYPCT LRQYLCVNTP SPRLAAMMLL QLLEGVDHLV QQGIAHRDLK SDNILVELDP DGCPWLVIAD FGCCLADESI GLQLPFSSWY VDRGGNGCLM APEVSTARPG PRAVIDYSKA DAWAVGAIAY EIFGLVNPFY GQGKAHLESR SYQEAQLPAL PESVPPDVRQ LVRALLQREA SKRPSARVAA NVL
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分子量
38 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PINK1 (PTEN-induced putative kinase 1) is a serine/threonine kinase that plays a crucial role in mitochondrial quality control and the regulation of cell death pathways. Mutations in the PINK1 gene are associated with autosomal recessive forms of Parkinson's disease, highlighting its significance in neurodegenerative disorders. PINK1 functions by sensing mitochondrial dysfunction and initiating protective mechanisms, including the activation of the Parkin protein, which tags damaged mitochondria for degradation through mitophagy. The study of recombinant PINK1 protein has gained momentum due to its potential therapeutic implications and the need for a better understanding of its enzymatic activity and interaction with other mitochondrial proteins. Researchers are focused on overexpressing and purifying PINK1 in various systems to study its structure, function, and biochemical characteristics. This research is essential for elucidating the molecular mechanisms underlying PINK1-related pathologies and may lead to novel strategies for the treatment of Parkinson's disease and other mitochondrial dysfunction-associated conditions. By understanding the biological pathways mediated by PINK1, scientists aim to develop targeted therapies that can enhance mitochondrial function and protect neuronal health.
