Analytical Data
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基因名
PAICS
- Application
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别名
PAICS;ADE2;AIRC;PAIS;Bifunctional phosphoribosylaminoimidazole carboxylase/phosphoribosylaminoimidazole succinocarboxamide synthetase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P22234
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表达区间
1-425aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSMATAEVL NIGKKLYEGK TKEVYELLDS PGKVLLQSKD QITAGNAARK NHLEGKAAIS NKITSCIFQLLQEAGIKTAF TRKCGETAFI APQCEMIPIE WVCRRIATGS FLKRNPGVKE GYKFYPPKVE LFFKDDANND PQWSEEQLIA AKFCFAGLLI GQTEVDIMSH ATQAIFEILE KSWLPQNCTL VDMKIEFGVD VTTKEIVLAD VIDNDSWRLW PSGDRSQQKD KQSYRDLKEV TPEGLQMVKK NFEWVAERVE LLLKSESQCR VVVLMGSTSD LGHCEKIKKA CGNFGIPCEL RVTSAHKGPD ETLRIKAEYE GDGIPTVFVA VAGRSNGLGP VMSGNTAYPV ISCPPLTPDW GVQDVWSSLR LPSGLGCSTV LSPEGSAQFA AQIFGLSNHL VWSKLRASIL NTWISLKQAD KKIRECNL
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分子量
50 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PAICS (Phosphoribosylaminoimidazole Carboxylase) is a crucial enzyme involved in the de novo purine biosynthesis pathway, which plays a significant role in producing nucleotides necessary for DNA and RNA synthesis. The study of PAICS has garnered attention due to its essential function in cellular metabolism, especially in rapidly dividing cells, such as cancer cells, where purine biosynthesis is often upregulated. Understanding the structure and function of PAICS at a molecular level can provide insights into the mechanisms of nucleotide metabolism and potential therapeutic targets for diseases characterized by dysregulated cell proliferation. In recent years, researchers have focused on the recombinant expression of PAICS to obtain large quantities of the enzyme for biochemical and structural studies. The development of effective expression systems, such as those utilizing E. coli or yeast, allows researchers to produce and purify PAICS for in-depth analysis. By employing techniques like X-ray crystallography and cryo-electron microscopy, scientists aim to elucidate the three-dimensional structure of PAICS, revealing its active sites and potential inhibitor binding pockets. This research not only enhances our understanding of purine metabolism but also aids in the design of small molecule inhibitors that could serve as novel cancer therapeutics, targeting the metabolic vulnerabilities of tumor cells. Thus, the study of recombinant PAICS is a pivotal area of research with implications for both basic biology and therapeutic applications.
