Analytical Data
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基因名
FPGS
- Application
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别名
FPGS；Folylpolyglutamate synthase. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q05932
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表达区间
1-587aa
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氨基酸序列
MSRARSHLRAALFLAAASARGITTQVAARRGLSAWPVPQEPSMEYQDAVRMLNTLQTNAGYLEQVKRQRGDPQTQLEAMELYLARSGLQVEDLDRLNIIHVTGTKGKGSTCAFTECILRSYGLKTGFFSSPHLVQVRERIRINGQPISPELFTKYFWRLYHRLEETKDGSCVSMPPYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAYDCTNIIRKPVVCGVSSLGIDHTSLLGDTVEKIAWQKGGIFKQGVPAFTVLQPEGPLAVLRDRAQQISCPLYLCPMLEALEEGGPPLTLGLEGEHQRSNAALALQLAHCWLQRQDRHGAGEPKASRPGLLWQLPLAPVFQPTSHMRLGLRNTEWPGRTQVLRRGPLTWYLDGAHTASSAQACVRWFRQALQGRERPSGGPEVRVLLFNATGDRDPAALLKLLQPCQFDYAVFCPNLTEVSSTGNADQQNFTVTLDQVLLRCLEHQQHWNHLDEEQASPDLWSAPSPEPGGSASLLLAPHPPHTCSASSLVFSCISHALQWISQGRDPIFQPPSPPKGLLTHPVAHSGASILREAAAIHVLVTGSLHLVGGVLKLLEPALSQ
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分子量
64.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
FPGS (folylpolyglutamate synthetase) is an essential enzyme involved in the metabolism of folate, a critical vitamin that plays a fundamental role in DNA synthesis, repair, and methylation processes. Research into FPGS has gained traction due to its implications in cancer biology and the potential development of therapeutics targeting folate metabolism. Malignant cells often exhibit altered folate pathways, leading to changes in cell proliferation and survival. By investigating FPGS at the molecular level, researchers aim to understand the enzyme’s regulation, structure, and function, and how these factors contribute to tumorigenesis. Furthermore, FPGS is a target for designing folate-based drug delivery systems, enhancing the efficacy of chemotherapy by selectively delivering cytotoxic agents to cancer cells while minimizing effects on healthy tissues. Studies have demonstrated that overexpression of FPGS can confer resistance to certain chemotherapy drugs, indicating its role in drug response and resistance mechanisms. This dual role of FPGS in both metabolic pathways and therapeutic implications underscores the biological significance of this enzyme. Thus, the study of recombinant FPGS through genetic engineering techniques is crucial for elucidating its function, developing new cancer treatment strategies, and improving patient outcomes in oncology.
