Analytical Data
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Gene name
MCOLN1
- Application
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Alternative Names
MCOLN1；ML4；TRPML1；Mucolipin-1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9GZU1
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Expression Region
1-580aa
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AA Sequence
MTAPAGPRGSETERLLTPNPGYGTQAGPSPAPPTPPEEEDLRRRLKYFFMSPCDKFRAKGRKPCKLMLQVVKILVVTVQLILFGLSNQLAVTFREENTIAFRHLFLLGYSDGADDTFAAYTREQLYQAIFHAVDQYLALPDVSLGRYAYVRGGGDPWTNGSGLALCQRYYHRGHVDPANDTFDIDPMVVTDCIQVDPPERPPPPPSDDLTLLESSSSYKNLTLKFHKLVNVTIHFRLKTINLQSLINNEIPDCYTFSVLITFDNKAHSGRIPISLETQAHIQECKHPSVFQHGDNSFRLLFDVVVILTCSLSFLLCARSLLRGFLLQNEFVGFMWRQRGRVISLWERLEFVNGWYILLVTSDVLTISGTIMKIGIEAKNLASYDVCSILLGTSTLLVWVGVIRYLTFFHNYNILIATLRVALPSVMRFCCCVAVIYLGYCFCGWIVLGPYHVKFRSLSMVSECLFSLINGDDMFVTFAAMQAQQGRSSLVWLFSQLYLYSFISLFIYMVLSLFIALITGAYDTIKHPGGAGAEESELQAYIAQCQDSPTSGKFRRGSGSACSLLCCCGRDPSEEHSLLVN
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Molecular Weight
66.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MCOLN1, or mucolipin-1, is a member of the transient receptor potential (TRP) ion channel family, which plays a crucial role in cellular processes, particularly in lysosomal function and calcium homeostasis. Mutations in the MCOLN1 gene are associated with mucolipidosis type IV (ML IV), a severe genetic disorder characterized by neurodevelopmental impairment and early-onset glaucoma, highlighting its importance in human health. Research into MCOLN1 recombinant proteins provides valuable insights into the molecular mechanisms underlying ML IV and the lysosomal dysfunction observed in affected individuals. By expressing and purifying the MCOLN1 protein, scientists can analyze its biochemical properties, interactions with other cellular components, and its ion channel activity. This information is essential for understanding how mutations disrupt function and lead to disease. Furthermore, exploring MCOLN1's role in cellular signaling pathways can offer potential therapeutic targets for ML IV and related lysosomal disorders. The advancements in recombinant protein technology and techniques such as cryo-electron microscopy enable a more detailed characterization of MCOLN1 structure and function, paving the way for drug development and innovative therapies aimed at mitigating the effects of MCOLN1-related pathologies. Overall, the study of MCOLN1 recombinant proteins not only enriches our understanding of lysosomal biology but also fosters the hope of improved treatment strategies for individuals suffering from genetic lysosomal storage diseases.
