Analytical Data
-
Gene name
PPP5C
- Application
-
Alternative Names
PPP5C；PPP5；Serine/threonine-Protein phosphatase 5
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P53041
-
Expression Region
2-499aa
-
AA Sequence
AMAEGERTECAEPPRDEPPADGALKRAEELKTQANDYFKAKDYENAIKFYSQAIELNPSNAIYYGNRSLAYLRTECYGYALGDATRAIELDKKYIKGYYRRAASNMALGKFRAALRDYETVVKVKPHDKDAKMKYQECNKIVKQKAFERAIAGDEHKRSVVDSLDIESMTIEDEYSGPKLEDGKVTISFMKELMQWYKDQKKLHRKCAYQILVQVKEVLSKLSTLVETTLKETEKITVCGDTHGQFYDLLNIFELNGLPSETNPYIFNGDFVDRGSFSVEVILTLFGFKLLYPDHFHLLRGNHETDNMNQIYGFEGEVKAKYTAQMYELFSEVFEWLPLAQCINGKVLIMHGGLFSEDGVTLDDIRKIERNRQPPDSGPMCDLLWSDPQPQNGRSISKRGVSCQFGPDVTKAFLEENNLDYIIRSHEVKAEGYEVAHGGRCVTVFSAPNYCDQMGNKASYIHLQGSDLRPQFHQFTAVPHPNVKPMAYANTLLQLGMM
-
Molecular Weight
64.2 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PPP5C, or protein phosphatase 5 catalytic subunit, is a member of the PP2C family of serine/threonine phosphatases, which play crucial roles in various cellular processes by regulating protein phosphorylation states. Research on PPP5C has gained traction due to its involvement in critical functions such as cell cycle regulation, stress response, and signal transduction pathways, particularly those related to cancer and neurodegenerative diseases. The enzyme is known to interact with numerous substrates, including kinases, which are pivotal in controlling cell proliferation and apoptosis. Its dysregulation has been implicated in several pathological conditions, making PPP5C a potential therapeutic target. Given its significance, researchers have focused on the structural and functional characterization of recombinant PPP5C to understand its enzymatic mechanisms better and to explore inhibitors that may modulate its activity. This research effort aims to deepen our understanding of the role of PPP5C in cellular signaling and its potential impact on disease treatment strategies. Through the development of recombinant PPP5C proteins, scientists can further investigate the enzyme's biochemical properties and its interactions with other cellular components, paving the way for innovative therapeutic approaches targeting this phosphatase.
