Analytical Data
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Gene name
DNASEII
- Application
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Alternative Names
DNASEII；DNASE2A；DNL2；Deoxyribonuclease-2-alpha
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O00115
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Expression Region
1-360aa
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AA Sequence
MIPLLLAALLCVPAGALTCYGDSGQPVDWFVVYKLPALRGSGEAAQRGLQ YKYLDESSGGWRDGRALINSPEGAVGRSLQPLYRSNTSQLAFLLYNDQPP QPSKAQDSSMRGHTKGVLLLDHDGGFWLVHSVPNFPPPASSAAYSWPHSA CTYGQTLLCVSFPFAQFSKMGKQLTYTYPWVYNYQLEGIFAQEFPDLENV VKGHHVSQEPWNSSITLTSQAGAVFQSFAKFSKFGDDLYSGWLAAALGTN LQVQFWHKTVGILPSNCSDIWQVLNVNQIAFPGPAGPSFNSTEDHSKWCV SPKGPWTCVGDMNRNQGEEQRGGGTLCAQLPALWKAFQPLVKNYQPCNGM ARKPSRAYKI
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Molecular Weight
66 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DNase II is an enzyme that plays a crucial role in the degradation of extracellular deoxyribonucleic acid (DNA) in various biological contexts, including cellular turnover and immune response. This endonuclease is particularly essential for the processing of apoptotic cells, as it facilitates the digestion of DNA released during programmed cell death, thereby preventing potential inflammatory responses and autoimmune diseases. Research into recombinant DNase II has gained traction due to its therapeutic potential; for instance, recombinant forms of this enzyme are being explored for their utility in clearing nucleic acids from the bloodstream in conditions characterized by excessive cell death or during certain viral infections. Furthermore, understanding the structural and functional properties of DNase II can provide insights into its mechanism of action and its interaction with substrates, paving the way for the development of enzyme inhibitors or activators that could be beneficial in treating diseases associated with aberrant DNA accumulation. Several studies have focused on the cloning, expression, and purification of DNase II, employing various expression systems to produce active recombinant proteins for functional assays. As our understanding of the enzyme’s role in both health and disease deepens, DNase II continues to be a promising target in the field of biomedicine, with ongoing research aimed at unlocking its full therapeutic potential.
