Analytical Data
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Gene name
MGMT
- Application
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Alternative Names
MGMT;Methylated-DNA--Protein-cysteine methyltransferase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P16455
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Expression Region
1-207aa
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AA Sequence
MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYF HQPEAIEEFP VPALHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN
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Molecular Weight
25 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MGMT (O6-methylguanine-DNA methyltransferase) is a crucial DNA repair protein involved in the direct reversal of DNA damage caused by alkylating agents, which are commonly found in various environmental toxins and certain chemotherapeutic drugs like temozolomide. The research into MGMT has gained substantial attention due to its significant role in cancer biology, particularly in the context of glioblastoma and other malignancies where alkylating agents are utilized as treatment options. The activity of MGMT is associated with resistance to these therapies, as high levels of MGMT expression can lead to the effective repair of drug-induced DNA damage, resulting in treatment failure. Consequently, understanding the structural and functional dynamics of MGMT has become paramount for developing therapeutic strategies that can either inhibit its action or predict patient response to alkylating agent-based treatments. Recent studies have focused on the protein’s mechanism of action, regulation, and its potential as a biomarker for treatment outcomes. The exploration of MGMT’s structure and the effects of genetic polymorphisms on its activity have opened new avenues for targeted interventions and personalized medicine approaches in oncology, emphasizing the importance of MGMT not only in cancer treatment efficacy but also in the broader scope of DNA damage response and repair mechanisms.
