Analytical Data
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Gene name
TPSd1
- Application
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Alternative Names
TPSd1；Tryptase delta
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BZJ3
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Expression Region
38-242aa
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AA Sequence
IVG GQEAPRSKWP WQVSLRVRGP YWMHFCGGSL IHPQWVLTAA HCVEPDIKDL AALRVQLREQ HLYYQDQLLP VSRIIVHPQF YIIQTGADIA LLELEEPVNI SSHIHTVTLP PASETFPPGM PCWVTGWGDV DNNVHLPPPY PLKEVEVPVV ENHLCNAEYH TGLHTGHSFQ IVRDDMLCAG SENHDSCQGD SGGPLVCKVN GT
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Molecular Weight
26.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The TPSd1 protein, derived from the thermophilic bacterium Thermus thermophilus, has emerged as a focal point of research due to its unique structural and functional characteristics. This protein is a member of the trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase (TPS) family, which play crucial roles in carbohydrate metabolism, particularly in the biosynthesis of trehalose. Trehalose is a disaccharide that serves as an important energy source and stress protectant in various organisms, particularly extremophiles like T. thermophilus. Understanding the enzymatic mechanisms of TPSd1 is significant for both fundamental science and practical applications, such as biotechnology and pharmaceuticals, due to the potential of trehalose in enhancing stress tolerance in crops and its protective properties in various industrial processes. Research efforts have focused on elucidating the structural dynamics and catalytic mechanisms of TPSd1, aiming to leverage its properties for biotechnological innovations. Furthermore, TPSd1's resilience at high temperatures offers insights into enzyme stability, which is critical in industrial applications requiring robust enzymatic activity under harsh conditions. Overall, the study of TPSd1 is paving the way for advancements in metabolic engineering and the development of novel bioproducts, thereby highlighting its importance in both environmental and commercial contexts.
