Analytical Data
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Gene name
KRT83
- Application
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Alternative Names
Hair keratin K2.10; Hard keratin; type II; 3; HB 3 ; HB3 ; hHb3 ; K2.10; K83; Keratin 83; Keratin; Keratin; hair; basic; 3; Keratin-83; Krt83; KRT83_HUMAN; KRTHB3 ; type II cuticular Hb3; Type II hair keratin Hb3; Type-II keratin Kb23
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P78385
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Expression Region
1-493aa
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AA Sequence
MTCGFNSIGCGFRPGNFSCVSACGPRPSRCCITAAPYRGISCYRGLTGGFGSHSVCGGFRAGSCGRSFGYRSGGVCGPSPPCITTVSVNESLLTPLNLEIDPNAQCVKQEEKEQIKSLNSRFAAFIDKVRFLEQQNKLLETKLQFYQNRECCQSNLEPLFAGYIETLRREAECVEADSGRLASELNHVQEVLEGYKKKYEEEVALRATAENEFVALKKDVDCAYLRKSDLEANVEALIQEIDFLRRLYEEEIRILQSHISDTSVVVKLDNSRDLNMDCIVAEIKAQYDDIATRSRAEAESWYRSKCEEMKATVIRHGETLRRTKEEINELNRMIQRLTAEVENAKCQNSKLEAAVAQSEQQGEAALSDARCKLAELEGALQKAKQDMACLIREYQEVMNSKLGLDIEIATYRRLLEGEEQRLCEGVEAVNVCVSSSRGGVVCGDLCVSGSRPVTGSVCSAPCNGNLVVSTGLCKPCGQLNTTCGGGSCGQGRH
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Molecular Weight
80.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
KRT83 is a member of the keratin family, specifically classified within the type I acidic keratins. Keratins are essential intermediate filament proteins that play a crucial role in the structural integrity and resilience of epithelial cells. Research on KRT83 has garnered interest due to its potential implications in skin biology, wound healing, and the pathology of various skin disorders. Mutations or dysregulation of keratin genes are often linked to conditions such as epidermolysis bullosa and other keratinization disorders. Additionally, KRT83 is involved in the formation of the cornified envelope, an essential barrier that protects against environmental stressors. Understanding the expression patterns and functional roles of KRT83 could provide insights into epidermal differentiation and repair processes, contributing to the development of targeted therapeutic strategies for skin-related diseases. Recently, advances in recombinant protein technology have facilitated the production and study of KRT83, allowing researchers to investigate its properties, interactions, and potential clinical applications. This research not only enhances our understanding of keratin biology but also paves the way for innovative approaches in regenerative medicine and dermatology. Overall, the study of KRT83 and its recombinant forms represents a promising avenue for elucidating the complex mechanisms of skin health and disease.
