Analytical Data
-
Gene name
KCNN3
- Application
-
Alternative Names
KCNN3; K3; Small conductance calcium-activated potassium channel protein 3; SK3; SKCa 3; SKCa3; KCa2.3
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q9UGI6
-
Expression Region
1-426aa
-
AA Sequence
MERPIKDSMFSLALKCLISLSTIILLGLIIAYHTREVQLFVIDNGADDWRIAMTYERILYISLEMLVCAIHPIPGEYKFFWTARLAFSYTPSRAEADVDIILSIPMFLRLYLIARVMLLHSKLFTDASSRSIGALNKINFNTRFVMKTLMTICPGTVLLVFSISLWIIAAWTVRVCERYHDQQDVTSNFLGAMWLISITFLSIGYGDMVPHTYCGKGVCLLTGIMGAGCTALVVAVVARKLELTKAEKHVHNFMMDTQLTKRIKNAAANVLRETWLIYKHTKLLKKIDHAKVRKHQRKFLQAIHQLRSVKMEQRKLSDQANTLVDLSKMQNVMYDLITELNDRSEDLEKQIGSLESKLEHLTASFNSLPLLIADTLRQQQQQLLSAIIEARGVSVAVGTTHTPISDSPIGVSSTSFPTPYTSSSSC
-
Molecular Weight
72.6 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
KCNN3, also known as the calcium-activated potassium channel KCa3.1, plays a critical role in various physiological processes, including cell proliferation, migration, and apoptosis. This channel is widely expressed in immune cells, endothelial cells, and smooth muscle cells, making it a candidate for therapeutic interventions in conditions such as hypertension, cancer, and autoimmune diseases. The research on KCNN3 recombinant proteins aims to elucidate the structure-function relationship of this channel and its regulatory mechanisms. Understanding these aspects is crucial for the development of selective channel modulators that can either inhibit or enhance channel activity to treat specific disorders. Furthermore, KCNN3's involvement in important signaling pathways, particularly those related to calcium homeostasis and cell activation, underscores the need to investigate its pharmacological potential. Recent studies have focused on characterizing KCNN3 through recombinant technology, allowing for high-yield production and purification of the protein. These recombinant proteins serve as valuable tools for functional assays and structural studies, enabling researchers to explore channel kinetics, ion selectivity, and gating mechanisms. Moreover, the generation of specific KCNN3 inhibitors and activators can provide insights into its biological roles and therapeutic applications, highlighting its significance in both basic and translational research. Thus, the study of KCNN3 and its recombinant forms presents a promising avenue for discovering novel treatments targeting a wide range of diseases linked to calcium signaling and ion channel dysregulation.
