Analytical Data
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Gene name
HtrA2/Omi
- Application
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Alternative Names
HTRA2;OMI;PRSS25;Serine protease HTRA2. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O43464
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Expression Region
133-358aa
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AA Sequence
AAVPSPPP ASPRSQYNFI ADVVEKTAPA VVYIEILDRH PFLGREVPIS NGSGFVVAAD GLIVTNAHVV ADRRRVRVRL LSGDTYEAVV TAVDPVADIA TLRIQTKEPL PTLPLGRSAD VRQGEFVVAM GSPFALQNTI TSGIVSSAQR PARDLGLPQT NVEYIQTDAA IDFGNSGGPL VNLDGEVIGV NTMKVTAGIS FAIPSDRLRE FLHRGEKKNS SSGISGSQ
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Molecular Weight
29 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HtrA2/Omi is a serine protease that plays a crucial role in cellular processes, including apoptosis and mitochondrial function. Originally identified in the context of neurodegenerative diseases, such as Parkinson's, HtrA2/Omi is released from the mitochondria in response to cellular stress and has been implicated in caspase-independent apoptotic pathways. Its main function involves the degradation of misfolded proteins, helping to maintain proteostasis within the cell. Additionally, HtrA2/Omi acts as a key player in the regulation of inflammation and mitochondrial homeostasis, making it an important factor in various pathological conditions, including cancer and neurodegeneration. The study of recombinant HtrA2/Omi protein has gained momentum, as understanding its structure and function could lead to therapeutic advancements. By utilizing recombinant techniques, researchers aim to explore HtrA2/Omi's enzymatic activity, substrate specificity, and interactions with other cellular proteins. This research not only enhances our understanding of fundamental biological processes but also holds potential for developing targeted therapies that can modulate HtrA2/Omi activity in disease states. Overall, the ongoing investigation into HtrA2/Omi contributes to a deeper comprehension of its multifaceted roles in health and disease, highlighting its significance as a target for future therapeutic strategies.
