Analytical Data
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基因名
AADAT
- Application
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别名
AADAT；KAT2；KYAT2；Kynurenine/alpha-aminoadipate aminotransferase. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8N5Z0
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表达区间
30-425aa
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氨基酸序列
PKSMISLAGGLPNPNMFPFKTAVITVENGKTIQFGEEMMKRALQYSPSAGIPELLSWLKQLQIKLHNPPTIHYPPSQGQMDLCVTSGSQQGLCKVFEMIINPGDNVLLDEPAYSGTLQSLHPLGCNIINVASDESGIVPDSLRDILSRWKPEDAKNPQKNTPKFLYTVPNGNNPTGNSLTSERKKEIYELARKYDFLIIEDDPYYFLQFNKFRVPTFLSMDVDGRVIRADSFSKIISSGLRIGFLTGPKPLIERVILHIQVSTLHPSTFNQLMISQLLHEWGEEGFMAHVDRVIDFYSNQKDAILAAADKWLTGLAEWHVPAAGMFLWIKVKGINDVKELIEEKAVKMGVLMLPGNAFYVDSSAPSPYLRASFSSASPEQMDVAFQVLAQLIKESL
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分子量
60.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AADAT (Aminoadipate aminotransferase) is a pivotal enzyme involved in the lysine degradation pathway, which plays a crucial role in amino acid metabolism. Recent research has highlighted the significance of AADAT in various physiological processes, including neurotransmitter synthesis and cellular signaling. Its dysfunction has been linked to neurological disorders and metabolic dysregulation, prompting scientists to investigate its structural and functional characteristics. The study of AADAT's recombinant protein is crucial for understanding its enzymatic mechanisms and interactions at a molecular level. By producing AADAT in a recombinant system, researchers aim to obtain sufficient quantities of the enzyme for detailed kinetic analyses and structural studies. This could ultimately lead to insights into its role in health and disease, with potential applications in developing therapeutic strategies targeting related metabolic disorders. Moreover, elucidating the three-dimensional structure of AADAT through methods like X-ray crystallography would provide valuable information about its active site and substrate specificity, thus contributing to the bioengineering of enzymes with tailored functions for industrial applications. Overall, the research into AADAT recombination protein aligns with the broader goal of understanding complex metabolic pathways and their implications in human health.
