Analytical Data
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Gene name
HS3ST1
- Application
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Alternative Names
HS3ST1;3OST;3OST1;Heparan sulfate glucosamine 3-O-sulfotransferase 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O14792
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Expression Region
21-307aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSRPAELGQ QELLRKAGTL QDDVRDGVAP NGSAQQLPQT IIIGVRKGGT RALLEMLSLH PDVAAAENEV HFFDWEEHYS HGLGWYLSQM PFSWPHQLTV EKTPAYFTSP KVPERVYSMN PSIRLLLILR DPSERVLSDY TQVFYNHMQK HKPYPSIEEF LVRDGRLNVD YKALNRSLYH VHMQNWLRFF PLRHIHIVDG DRLIRDPFPE IQKVERFLKL SPQINASNFY FNKTKGFYCL RDSGRDRCLH ESKGRAHPQV DPKLLNKLHE YFHEPNKKFF ELVGRTFDWH
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Molecular Weight
36 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HS3ST1, or Heparan Sulfate 3-O-Sulfotransferase 1, is an essential enzyme involved in the biosynthesis of heparan sulfate (HS), a glycosaminoglycan that plays critical roles in various biological processes, including cell signaling, development, and tissue repair. HS3ST1 specifically adds sulfate groups to the 3-O position of certain glucosamine residues in heparan sulfate chains, influencing the binding and activity of numerous growth factors, cytokines, and morphogens. Dysregulation of HS synthesis, including anomalies in HS3ST1, has been implicated in various pathologies, such as cancer, inflammation, and congenital disorders, making it a significant target for therapeutic intervention. Recent studies have focused on the structural and functional characterization of HS3ST1, revealing its intricate mechanisms and the effects of specific mutations on its activity. Understanding HS3ST1's role in heparan sulfate modification provides insights into its involvement in disease processes and highlights its potential as a biomarker and therapeutic target. This research is crucial for developing strategies to manipulate HS pathways for therapeutic purposes, with the aim of improving treatment outcomes in diseases associated with aberrant HS signaling.
