Analytical Data
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Gene name
HEXA
- Application
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Alternative Names
HEXA;Beta-hexosaminidase subunit alpha
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P06865
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Expression Region
1-529aa
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AA Sequence
MTSSRLWFSLLLAAAFAGRATALWPWPQNFQTSDQRYVLYPNNFQFQYDV S SAAQPGCSVLDEAFQRYRDLLFGSGSWPRPYLTGKRHTLEKNVLVVS VVTPGCNQLPTLESVENYTLTINDDQCLLLSETVWGALRGLETFSQLVWK SAEGTFFINKTEIEDFPRFPHRGLLLDTSRHYLPLSSILDTLDVMAYNKL NVFHWHLVDDPSFPYESFTFPELMRKGSYNPVTHIYTAQDVKEVIEYARL RGIRVLAEFDTPGHTLSWGPGIPGLLTPCYSGSEPSGTFGPVNPSLNNTY EFMSTFFLEVSSVFPDFYLHLGGDEVDFTCWKSNPEIQDFMRKKGFGEDF KQLESFYIQTLLDIVSSYGKGYVVWQEVFDNKVKIQPDTIIQVWREDIPV NYMKELELVTKAGFRALLSAPWYLNRISYGPDWKDFYIVEPLAFEGTPEQ KALVIGGEACMWGEYVDNTNLVPRLWPRAGAVAERLWSNKLTSDLTFAYE RLSHFRCELLRRGVQAQPLNVGFCEQEFEQTDYKDDDDKHHHHHH
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Molecular Weight
63 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
HEXA, a gene encoding the alpha subunit of the enzyme Hexosaminidase A, plays a critical role in lysosomal function by catalyzing the breakdown of GM2 gangliosides into GM3 gangliosides. Mutations in the HEXA gene lead to the deficiency of Hexosaminidase A, resulting in the accumulation of GM2 gangliosides, which is the pathological basis for Tay-Sachs disease, a severe neurodegenerative disorder characterized by progressive neurological impairment and early childhood mortality. Research on HEXA recombinant proteins has gained significant momentum as scientists aim to develop potential therapeutic strategies to address this genetic disorder. By producing functional HEXA proteins through recombinant DNA technology, researchers can explore enzyme replacement therapies and gene therapies to restore enzymatic activity in affected individuals. Additionally, the study of HEXA and its associated pathways holds promise for understanding broader lysosomal storage disorders and developing treatment modalities. Advances in structural biology and protein engineering are further enhancing our ability to create optimized HEXA proteins that could improve stability and efficiency, paving the way for innovative approaches to managing Tay-Sachs disease and potentially other similar lysosomal disorders.
