Analytical Data
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基因名
HS3ST2
- Application
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别名
HS3ST2; 3OST2; UNQ2442/PRO5004Heparan sulfate glucosamine 3-O-sulfotransferase 2; EC 2.8.2.29; Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 2; 3-OST-2; Heparan sulfate 3-O-sulfotransferase 2; h3-OST-2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9Y278
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表达区间
1-367aa
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氨基酸序列
MAYRVLGRAGPPQPRRARRLLFAFTLSLSCTYLCYSFLCCCDDLGRSRLLGAPRCLRGPSAGGQKLLQKSRPCDPSGPTPSEPSAPSAPAAAVPAPRLSGSNHSGSPKLGTKRLPQALIVGVKKGGTRAVLEFIRVHPDVRALGTEPHFFDRNYGRGLDWYRSLMPRTLESQITLEKTPSYFVTQEAPRRIFNMSRDTKLIVVVRNPVTRAISDYTQTLSKKPDIPTFEGLSFRNRTLGLVDVSWNAIRIGMYVLHLESWLQYFPLAQIHFVSGERLITDPAGEMGRVQDFLGIKRFITDKHFYFNKTKGFPCLKKTESSLLPRCLGKSKGRTHVQIDPEVIDQLREFYRPYNIKFYETVGQDFRWE
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分子量
41.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HS3ST2 (heparan sulfate 3-O-sulfotransferase 2) is an important enzyme involved in the biosynthesis of heparan sulfate, a polysaccharide that plays critical roles in various biological processes, including cell signaling, development, and tissue homeostasis. Research has shown that HS3ST2 is responsible for the specific sulfation of heparan sulfate chains, which modulates their interaction with a wide range of growth factors, cytokines, and extracellular matrix components. This enzymatic activity is particularly significant in regulating key signaling pathways, such as those involving fibroblast growth factor (FGF) and hepatocyte growth factor (HGF), which are essential for processes like angiogenesis and cell proliferation. Dysregulation of HS3ST2 has been implicated in various pathological conditions, including cancer, where altered heparan sulfate sulfation patterns can affect tumor growth and metastasis. Recent studies have focused on the recombinant production and characterization of HS3ST2 to better understand its functional roles and potential as a therapeutic target. By producing HS3ST2 as a recombinant protein, researchers aim to investigate its structural properties, enzymatic activity, and interactions with heparan sulfate substrates, paving the way for innovative approaches to manipulate heparan sulfate biology in disease contexts. This research not only enhances our understanding of HS3ST2's function but also opens avenues for the development of novel strategies in regenerative medicine and cancer therapy, underscoring the importance of this enzyme in both health and disease.
