Analytical Data
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基因名
LOX5
- Application
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别名
LOX5；LOG5；Polyunsaturated fatty acid 5-lipoxygenase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P09917
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表达区间
1-674aa
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氨基酸序列
MPSYTVTVATGSQWFAGTDDYIYLSLVGSAGCSEKHLLDKPFYNDFERGAVDSYDVTVDEELGEIQLVRIEKRKYWLNDDWYLKYITLKTPHGDYIEFPCYRWITGDVEVVLRDGRAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVMNHWQEDLMFGYQFLNGCNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGIDANKTDPCTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGDENPIFLPSDAKYDWLLAKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKAREQLICECGLFDKANATGGGGHVQMVQRAMKDLTYASLCFPEAIKARGMESKEDIPYYFYRDDGLLVWEAIRTFTAEVVDIYYEGDQVVEEDPELQDFVNDVYVYGMRGRKSSGFPKSVKSREQLSEYLTVVIFTASAQHAAVNFGQYDWCSWIPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMARFRKNLEAIVSVIAERNKKKQLPYYYLSPDRIPNSVAI
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分子量
77.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The LOX5 (Lipoxygenase 5) enzyme is a crucial player in the metabolism of arachidonic acid, leading to the production of various bioactive lipid mediators, such as leukotrienes, which are significant in inflammatory responses. Research on LOX5 has gained momentum due to its implications in various pathological conditions, including asthma, cardiovascular diseases, and cancer. This enzyme's synthesis and regulation are complex and involve multiple isoforms and regulatory pathways, making it a target of interest for therapeutic intervention. The generation of recombinant LOX5 protein allows for detailed biochemical and biophysical studies, facilitating the understanding of its structure-function relationships and the modulation of its activity. By analyzing the recombinant protein, researchers aim to uncover the specific mechanisms through which LOX5 contributes to inflammatory and immune responses, potentially leading to the development of novel anti-inflammatory drugs. Moreover, the study of LOX5 and its associated signaling pathways could provide insights into the broader context of lipid mediator actions in health and disease, aiding in the identification of biomarkers for disease progression and therapeutic efficacy. Thus, investigating LOX5 at the molecular level through recombinant protein technologies serves as an essential step towards harnessing its potential in clinical applications.
