Analytical Data
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Gene name
iolO
- Application
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Alternative Names
iolO；5-keto-L-gluconate epimerase
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9WYP7
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Expression Region
1-270aa
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AA Sequence
MKLSLVISTSDAAFDALAFKGDLRKGMELAKRVGYQAVEIAVRDPSIVDWNEVKILSEELNLPICAIGTGQAYLADGLSLTHPNDEIRKKAIERVVKHTEVAGMFGALVIIGLVRGRREGRSYEETEELFIESMKRLLELTEHAKFVIEPLNRYETDFINTIDDALRILRKINSNRVGILADTFHMNIEEVNIPESLKRAGEKLYHFHVADSNRWAPGCGHFDFRSVFNTLKEIGYNRYVSVECLPLPGGMEEAAEIAFKTLKELIIKLT
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Molecular Weight
37.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of iolO recombinant protein is rooted in the increasing interest in microbial biotechnology and its potential applications in various industries. IolO is an enzyme encoded by the iolO gene, which is part of the iol operon involved in inositol catabolism in certain bacteria, particularly in the context of pathogenic species like Pseudomonas aeruginosa. Understanding the function and characteristics of iolO is significant because it plays a crucial role in the utilization of inositol, a common sugar alcohol that can impact bacterial growth and metabolism. Recombinant protein technology allows for the expression of iolO in heterologous systems, facilitating the study of its biochemical properties, structure, and interaction with other metabolic pathways. This research can reveal valuable insights into bacterial physiology and contribute to the development of biotechnological applications, such as enzymatic processes or the production of bioproducts. Furthermore, as antibiotic resistance becomes a growing concern, targeting bacterial metabolic pathways like those involving iolO may offer innovative strategies for combating infections. Overall, the investigation of iolO recombinant protein not only enhances our understanding of bacterial metabolism but also opens up avenues for practical applications in medicine and industry.
