Cat: PA2000-8344

Recombinant Human HPN Protein,GST

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Analytical Data

  • Gene name

    HPN

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    HPN; TMPRSS1; Serine protease hepsin; Transmembrane protease serine 1

  • Species

    Human

  • Source

    E. coli

  • Tag

    GST-tag at N-terminal

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    P05981

  • Expression Region

    40-417aa

  • AA Sequence

    VAVLLRSDQEPLYPVQVSSADARLMVFDKTEGTWRLLCSSRSNARVAGLSCEEMGFLRALTHSELDVRTAGANGTSGFFCVDEGRLPHTQRLLEVISVCDCPRGRFLAAICQDCGRRKLPVDRIVGGRDTSLGRWPWQVSLRYDGAHLCGGSLLSGDWVLTAAHCFPERNRVLSRWRVFAGAVAQASPHGLQLGVQAVVYHGGYLPFRDPNSEENSNDIALVHLSSPLPLTEYIQPVCLPAAGQALVDGKICTVTGWGNTQYYGQQAGVLQEARVPIISNDVCNGADFYGNQIKPKMFCAGYPEGGIDACQGDSGGPFACEDSISRTPRWRLCGIVSWGTGCALAQKPGVYTKVSDFREWIFQAIKTHSEASGMVTQL

  • Molecular Weight

    67.43 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

HPN (hepatocyte growth factor activator) is a serine protease that plays a crucial role in the activation of hepatocyte growth factor (HGF), which is involved in various physiological processes such as tissue regeneration, cell migration, and angiogenesis. The research into HPN has gained momentum due to its significant implications in cancer biology, particularly in the tumor microenvironment where it can influence tumor growth and metastasis. Previous studies have highlighted a correlation between HPN expression levels and poor prognosis in certain cancers, suggesting that HPN could serve as a potential biomarker for cancer progression. Furthermore, the therapeutic modulation of HPN activity may offer novel strategies for cancer treatment, making it a target of interest for drug development. Understanding the molecular mechanisms underlying HPN's role in cell signaling pathways is essential for elucidating its functions and therapeutic potential. As such, ongoing research aims to unravel the complexities of HPN activity, investigate its interactions with other proteins, and explore its potential applications in regenerative medicine and oncology. The exploration of recombinant HPN proteins has also opened new avenues for pharmaceutical applications, enhancing our understanding of its structure-function relationships and paving the way for innovative treatments targeting HPN-related diseases.

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