Analytical Data
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基因名
GTF2H5
- Application
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别名
GTF2H5;C6orf175;TTDA;General transcription factor IIH subunit 5
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q6ZYL4
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表达区间
1-71aa
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氨基酸序列
MVNVLKGVLIECDPAMKQFLLYLDESNALGKKFIIQDIDDTHVFVIAELVNVLQERVGELMDQNAFSLTQK
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分子量
35.1kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GTF2H5, a subunit of the general transcription factor IIH (TFIIH) complex, plays a crucial role in the regulation of gene expression and DNA repair mechanisms. TFIIH is a multi-protein complex involved in the transcription initiation of protein-coding genes and the nucleotide excision repair of DNA. GTF2H5 specifically contributes to the assembly of TFIIH and is essential for its helicase activity, which unwinds DNA strands during transcription. Dysregulation or mutations in GTF2H5 can lead to various diseases, including cancer and disorders related to DNA repair deficiencies. Research on the recombinant form of GTF2H5 focuses on its structural and functional analysis to better understand its role in transcription and DNA repair pathways. The recombinant protein can be used for biochemical assays, protein-protein interaction studies, and structural determinations, which are vital for elucidating the mechanisms of transcription regulation and the repair processes of damaged DNA. Understanding GTF2H5 at the molecular level may also provide insights into therapeutic targets for diseases associated with its dysfunction, making it an important focus within molecular biology and genetics research.
