Analytical Data
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Gene name
GRK1
- Application
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Alternative Names
EC=2.7.11.14; G protein-coupled receptor kinase 1; GPRK1; Grk1; Rhodopsin kinase; RHOK; RK; RK_HUMAN
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q15835
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Expression Region
1-560aa
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AA Sequence
MDFGSLETVV ANSAFIAARG SFDGSSSQPS RDKKYLAKLK LPPLSKCESL RDSLSLEFES VCLEQPIGKK LFQQFLQSAE KHLPALELWK DIEDYDTADN DLQPQKAQTI LAQYLDPQAK LFCSFLDEGI VAKFKEGPVE IQDGLFQPLL QATLAHLGQA PFQEYLGSLY FLRFLQWKWL EAQPMGEDWF LDFRVLGKGG FGEVSACQMK ATGKLYACKK LNKKRLKKRK GYQGAMVEKK ILMKVHSRFI VSLAYAFETK ADLCLVMTIM NGGDIRYHIY NVNEENPGFP EPRALFYTAQ IICGLEHLHQ RRIVYRDLKP ENVLLDNDGN VRISDLGLAV ELLDGQSKTK GYAGTPGFMA PELLQGEEYD FSVDYFALGV TLYEMIAARG PFRARGEKVE NKELKHRIIS EPVKYPDKFS QASKDFCEAL LEKDPEKRLG FRDETCDKLR AHPLFKDLNW RQLEAGMLMP PFIPDSKTVY AKDIQDVGAF STVKGVAFDK TDTEFFQEFA TGNCPIPWQE EMIETGIFGE LNVWRSDGQM PDDMKGISGG SSSSSKSGMC
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Molecular Weight
63.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GRK1 (G protein-coupled receptor kinase 1) is a crucial enzyme primarily expressed in photoreceptor cells in the retina. It plays a significant role in the phosphorylation and desensitization of activated rhodopsin, a vital photoreceptor protein necessary for vision. The study of GRK1 recombinant protein is essential for understanding various pathological conditions, including retinitis pigmentosa and other retinal disorders, where GRK1 dysfunction contributes to vision loss. The production of GRK1 as a recombinant protein allows researchers to investigate its biochemical properties, regulatory mechanisms, and interactions with other proteins. By utilizing advanced techniques in protein expression and purification, scientists can produce active GRK1 for in vitro studies, which aids in elucidating its role in cellular signaling pathways and potential therapeutic targets. Furthermore, understanding GRK1's structure and function at a molecular level provides insights into the development of pharmacological agents that can modulate its activity, offering promising avenues for treating vision-related diseases. The exploration of GRK1 not only enhances our understanding of visual signal transduction but also contributes to broader research in receptor signaling and its implications in various health conditions. Overall, GRK1 remains a significant focus for researchers aiming to unravel the complexities of phototransduction and the potential for therapeutic interventions in retinal degenerative diseases.
