Analytical Data
-
基因名
DCTD
- Application
-
别名
DCTD;Deoxycytidylate deaminase
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P32321
-
表达区间
1-178aa
-
氨基酸序列
MGSSHHHHHHSSGLVPRGSHMSEVSCKKRDDYLEWPEYFMAVAFLSAQRS KDPNSQVGACIVNSENKIVGIGYNGMPNGCSDDVLPWRRTAENKLDTKYP YVCHAELNAIMNKNSTDVKGCSMYVALFPCNECAKLIIQAGIKEVIFMSD KYHDSDEATAARLLFNMAGVTFRKFIPKCSKIVIDFDSINSRPSQKLQ
-
分子量
22 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DCTD (deoxycytidine triphosphate diphosphohydrolase) is an essential enzyme involved in nucleotide metabolism, specifically in the hydrolysis of dCTP to dCDP and inorganic phosphate. This enzymatic reaction is crucial for maintaining the balance of deoxynucleotides within the cell, which is vital for DNA synthesis and repair pathways. Dysregulation of DCTD has been implicated in various diseases, including cancer, as altered nucleotide pools can lead to genomic instability. Understanding the structure and function of DCTD, as well as the mechanisms governing its activity, may provide insights for therapeutic interventions. Research on recombinant DCTD proteins offers a valuable avenue for characterizing the enzyme’s biochemical properties, elucidating its role in nucleotide metabolism, and exploring potential applications in drug development. By generating and studying DCTD in a controlled laboratory setting, scientists can investigate its interactions with other biomolecules, assess the impact of mutations, and identify small-molecule inhibitors that could modulate its activity. This research not only contributes to the fundamental understanding of cellular metabolism but also opens up possibilities for innovative approaches to treat diseases associated with nucleotide imbalances.
