Analytical Data
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基因名
RIPK1
- Application
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别名
RIPK1；RIP；RIP1；Receptor-interacting serine/threonine-Protein kinase 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q13546
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表达区间
1-671aa
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氨基酸序列
MQPDMSLNVI KMKSSDFLES AELDSGGFGK VSLCFHRTQG LMIMKTVYKG PNCIEHNEAL LEEAKMMNRL RHSRVVKLLG VIIEEGKYSL VMEYMEKGNL MHVLKAEMST PLSVKGRIIL EIIEGMCYLH GKGVIHKDLK PENILVDNDF HIKIADLGLA SFKMWSKLNN EEHNELREVD GTAKKNGGTL YYMAPEHLND VNAKPTEKSD VYSFAVVLWA IFANKEPYEN AICEQQLIMC IKSGNRPDVD DITEYCPREI ISLMKLCWEA NPEARPTFPG IEEKFRPFYL SQLEESVEED VKSLKKEYSN ENAVVKRMQS LQLDCVAVPS SRSNSATEQP GSLHSSQGLG MGPVEESWFA PSLEHPQEEN EPSLQSKLQD EANYHLYGSR MDRQTKQQPR QNVAYNREEE RRRRVSHDPF AQQRPYENFQ NTEGKGTAYS SAASHGNAVH QPSGLTSQPQ VLYQNNGLYS SHGFGTRPLD PGTAGPRVWY RPIPSHMPSL HNIPVPETNY LGNTPTMPFS SLPPTDESIK YTIYNSTGIQ IGAYNYMEIG GTSSSLLDST NTNFKEEPAA KYQAIFDNTT SLTDKHLDPI RENLGKHWKN CARKLGFTQS QIDEIDHDYE RDGLKEKVYQ MLQKWVMREG IKGATVGKLA QALHQCSRID LLSSLIYVSQ N
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分子量
108 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Receptor-interacting protein kinase 1 (RIPK1) plays a crucial role in the regulation of cell survival, apoptosis, and inflammation, making it a significant focus in cellular biology and therapeutic research. Originally identified as a key signaling molecule in tumor necrosis factor (TNF) receptor pathways, RIPK1 functions at the crossroads of cell death and survival mechanisms. Its ability to mediate necroptosis, a form of programmed cell death distinct from apoptosis, highlights its relevance in various pathophysiological conditions, including neurodegenerative diseases, inflammatory disorders, and cancer. The study of recombinant RIPK1 proteins has provided insights into its structure-function relationships, aiding in the elucidation of its regulatory roles in signaling pathways. Researchers have developed various recombinant techniques to express and purify RIPK1, allowing for detailed biochemical characterization and the exploration of its interactions with other cellular proteins. Furthermore, investigating RIPK1's phosphorylation states and other post-translational modifications offers potential avenues for targeted therapies. Given its involvement in critical cellular processes, understanding RIPK1 through recombinant protein studies is essential for developing novel interventions for diseases where dysregulation of cell death pathways occurs. This ongoing research has implications for drug design and the development of therapeutic strategies aimed at modulating RIPK1 activity, further underscoring its significance in health and disease.
