Analytical Data
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Gene name
CCNA2
- Application
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Alternative Names
CCNA2；CCN1；CCNA；Cyclin-A2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P20248
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Expression Region
1-432aa
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AA Sequence
MLGNSAPGPATREAGSALLALQQTALQEDQENINPEKAAPVQQPRTRAALAVLKSGNPRGLAQQQRPKTRRVAPLKDLPVNDEHVTVPPWKANSKQPAFTIHVDEAEKEAQKKPAESQKIEREDALAFNSAISLPGPRKPLVPLDYPMDGSFESPHTMDMSIILEDEKPVSVNEVPDYHEDIHTYLREMEVKCKPKVGYMKKQPDITNSMRAILVDWLVEVGEEYKLQNETLHLAVNYIDRFLSSMSVLRGKLQLVGTAAMLLASKFEEIYPPEVAEFVYITDDTYTKKQVLRMEHLVLKVLTFDLAAPTVNQFLTQYFLHQQPANCKVESLAMFLGELSLIDADPYLKYLPSVIAGAAFHLALYTVTGQSWPESLIRKTGYTLESLKPCLMDLHQTYLKAPQHAQQSIREKYKNSKYHGVSLLNPPETLNL
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Molecular Weight
54.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CCNA2, a member of the cyclin family, plays a crucial role in regulating the cell cycle, particularly the transition from the G1 phase to the S phase. Its importance is underscored by its involvement in various cellular processes, including DNA replication and cell division. Research into the recombinant expression of CCNA2 is driven by its potential implications in cancer biology, where dysregulation of the cell cycle is a hallmark of tumorigenesis. The ability to produce recombinant CCNA2 protein allows for the exploration of its functional properties, protein interactions, and the molecular mechanisms governing cell cycle progression. Moreover, studying CCNA2 in a controlled environment can provide insights into targeted therapies aimed at re-establishing normal cell cycle regulation in cancerous cells. As a consequence, ongoing research efforts focus on optimizing protocols for the recombinant production of CCNA2, using systems such as bacterial or yeast expression systems, to facilitate subsequent biochemical and structural studies. Understanding the structure-function relationship of CCNA2 in the context of the cell cycle not only enhances our knowledge of fundamental cellular mechanisms but also opens avenues for developing innovative therapeutic strategies in cancer treatment, making CCNA2 a significant target for further study in molecular and cellular biology.
