Analytical Data
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Gene name
OPTN
- Application
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Alternative Names
OPTN；FIP2；GLC1E；HIP7；Optineurin
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96CV9
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Expression Region
1-577aa
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AA Sequence
MSHQPLSCLTEKEDSPSESTGNGPPHLAHPNLDTFTPEELLQQMKELLTE NHQLKEAMKLNNQAMKGRFEELSAWTEKQKEERQFFEIQSKEAKERLMAL SHENEKLKEELGKLKGKSERSSEDPTDDSRLPRAEAEQEKDQLRTQVVRL QAEKADLLGIVSELQLKLNSSGSSEDSFVEIRMAEGEAEGSVKEIKHSPG PTRTVSTGTALSKYRSRSADGAKNYFEHEELTVSQLLLCLREGNQKVERL EVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEEKGPETVGSEVE ALNLQVTSLFKELQEAHTKLSKAELMKKRLQEKCQALERKNSAIPSELNE KQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVLQMTHNKLLQEH NNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALASKQLQMDEMKQT IAKQEEDLETMTILRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVLL KENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNI PIHSCPKCGEVLPDIDTLQIHVMDCII
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Molecular Weight
68 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The OPTN (optineurin) gene plays a crucial role in various cellular processes, including intracellular trafficking, autophagy, and immune response regulation. Initially identified for its mutations associated with glaucoma, recent studies have expanded its significance to neurodegenerative diseases, particularly amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Research has shown that misfolded or aggregates of OPTN can lead to cellular dysfunction and neuronal death. Furthermore, OPTN is involved in the regulation of NF-κB signaling and can modulate the inflammatory response, highlighting its potential role in neuroinflammation. Given its multifaceted functions, the study of OPTN-recombinant proteins has become pivotal for understanding its mechanisms in both health and disease. Investigating these proteins can shed light on the pathways contributing to neurodegeneration and may provide targets for therapeutic intervention. Additionally, OPTN's involvement in autophagy suggests that manipulating its activity could enhance the clearance of toxic protein aggregates. Overall, the exploration of OPTN-recombinant proteins is essential for unraveling the complexities of pathologies linked to this gene and developing innovative strategies to combat neurodegenerative disorders.
