Analytical Data
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基因名
ACSL4
- Application
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别名
ACSL4；ACS4；FACL4；LACS4；Long-chain-fatty-acid--CoA ligase 4
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O60488
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表达区间
1-711aa
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氨基酸序列
MKLKLNVLTIILLPVHLLITIYSALIFIPWYFLTNAKKKNAMAKRIKAKPTSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVSKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWMNYLEVNRRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKIGYDYKLEQIKKGYDAPLCNLLLFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTINDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKRLTLLAQQKGVEGTWVDICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELRNHYLKDIERMYGGK
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分子量
82.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Identification
Protein Description
ACSL4 (acyl-CoA synthetase long-chain family member 4) is a key enzyme involved in lipid metabolism, specifically in the activation of long-chain fatty acids for subsequent metabolic processes. The importance of ACSL4 has gained recognition due to its diverse roles in cellular functions, including energy production, membrane synthesis, and signaling pathways. Research has increasingly focused on the implications of ACSL4 in various physiological and pathological contexts, particularly its involvement in cancer, neurodegenerative diseases, and metabolic disorders. Elevated expression levels of ACSL4 have been associated with aggressive tumor phenotypes, leading to interest in its potential as a therapeutic target or biomarker. Furthermore, the enzyme's role in the regulation of fatty acid oxidation and inflammation has positioned it as a significant player in understanding metabolic diseases, such as obesity and diabetes. The study of recombinant ACSL4 protein has become fundamental to elucidate its structure-function relationships, enabling the exploration of inhibitor development and therapeutic interventions. By generating and characterizing recombinant ACSL4, researchers aim to dissect its enzymatic mechanisms, interactions with other cellular components, and overall contribution to metabolic dysregulation. This research not only enhances our understanding of ACSL4's biological functions but also opens avenues for innovative strategies in treating related diseases. Thus, the investigation of ACSL4 presents a promising frontier in biochemical research, with implications for health and disease management.
