Analytical Data
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基因名
NAGS
- Application
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别名
NAGS；N-acetylglutamate synthase. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8N159
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表达区间
1-534aa
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氨基酸序列
MATALMAVVLRAAAVAPRLRGRGGTGGARRLSCGARRRAARGTSPGRRLSTAWSQPQPPPEEYAGADDVSQSPVAEEPSWVPSPRPPVPHESPEPPSGRSLVQRDIQAFLNQCGASPGEARHWLTQFQTCHHSADKPFAVIEVDEEVLKCQQGVSSLAFALAFLQRMDMKPLVVLGLPAPTAPSGCLSFWEAKAQLAKSCKVLVDALRHNAAAAVPFFGGGSVLRAAEPAPHASYGGIVSVETDLLQWCLESGSIPILCPIGETAARRSVLLDSLEVTASLAKALRPTKIIFLNNTGGLRDSSHKVLSNVNLPADLDLVCNAEWVSTKERQQMRLIVDVLSRLPHHSSAVITAASTLLTELFSNKGSGTLFKNAERMLRVRSLDKLDQGRLVDLVNASFGKKLRDDYLASLRPRLHSIYVSEGYNAAAILTMEPVLGGTPYLDKFVVSSSRQGQGSGQMLWECLRRDLQTLFWRSRVTNPINPWYFKHSDGSFSNKQWIFFWFGLADIRDSYELVNHAKGLPDSFHKPASDPGS
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分子量
58.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NAGS (N-acetylglutamate synthetase) is a crucial enzyme involved in the metabolism of amino acids, specifically playing a key role in the urea cycle by facilitating the synthesis of N-acetylglutamate, which is an essential activator of carbamoyl phosphate synthetase I. Disorders related to NAGS can lead to hyperammonemia, a potentially life-threatening condition. The study of NAGS recombinant protein is paramount for understanding its structure-function relationship and the underlying biological mechanisms. Advances in recombinant DNA technology enable the production of NAGS in host systems, providing a valuable tool for examining its enzymatic activity and regulatory mechanisms. Furthermore, characterizing NAGS through recombinant approaches can aid in the development of therapeutic strategies for treating metabolic disorders associated with its deficiency. Research efforts are focused on elucidating the enzyme's kinetics, stability, and interaction with other metabolic pathways, contributing to a deeper understanding of its role in amino acid metabolism and potential implications for gene therapy. Overall, the exploration of NAGS recombinant protein presents significant opportunities for both basic biochemistry and clinical applications in metabolic disease management.
