Analytical Data
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Gene name
GLIS1
- Application
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Alternative Names
GLIS1; Zinc finger protein GLIS1; GLI-similar 1
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8NBF1
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Expression Region
1-620aa
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AA Sequence
MAEARTSLSAHCRGPLATGLHPDLDLPGRSLATPAPSCYLLGSEPSSGLGLQPETHLPEGSLKRCCVLGLPPTSPASSSPCASSDVTSIIRSSQTSLVTCVNGLRSPPLTGDLGGPSKRARPGPASTDSHEGSLQLEACRKASFLKQEPADEFSELFGPHQQGLPPPYPLSQLPPGPSLGGLGLGLAGRVVAGRQACRWVDCCAAYEQQEELVRHIEKSHIDQRKGEDFTCFWAGCVRRYKPFNARYKLLIHMRVHSGEKPNKCMFEGCSKAFSRLENLKIHLRSHTGEKPYLCQHPGCQKAFSNSSDRAKHQRTHLDTKPYACQIPGCSKRYTDPSSLRKHVKAHSAKEQQVRKKLHAGPDTEADVLTECLVLQQLHTSTQLAASDGKGGCGLGQELLPGVYPGSITPHNGLASGLLPPAHDVPSRHHPLDATTSSHHHLSPLPMAESTRDGLGPGLLSPIVSPLKGLGPPPLPPSSQSHSPGGQPFPTLPSKPSYPPFQSPPPPPLPSPQGYQGSFHSIQSCFPYGDCYRMAEPAAGGDGLVGETHGFNPLRPNGYHSLSTPLPATGYEALAEASCPTALPQQPSEDVVSSGPEDCGFFPNGAFDHCLGHIPSIYTDT
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Molecular Weight
68.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GLIS1 (GLI-similar 1) is a transcription factor belonging to the GLI family, which plays a crucial role in various biological processes, including cell proliferation, differentiation, and development. Dysregulation of GLIS1 has been implicated in several diseases, particularly in the context of cancer and developmental disorders. Research into GLIS1 has gained momentum due to its potential as a therapeutic target and biomarker for disease progression. Recombinant GLIS1 protein studies are essential for understanding its biological functions, interaction with other proteins, and regulatory mechanisms. By generating recombinant GLIS1, researchers can investigate its role in signaling pathways, specifically in the Hedgehog signaling pathway, which is vital for embryonic development and tissue homeostasis. Additionally, the study of GLIS1's structural properties through recombinant technology enables the identification of binding sites and interaction domains, paving the way for the development of small molecules or peptides that could modulate its activity. Moreover, GLIS1's involvement in stem cell regulation and potential links to pluripotency make it a prominent focus in regenerative medicine research. As such, recombinant GLIS1 not only provides insights into its functional roles but also facilitates the exploration of its potential therapeutic applications, making it a significant subject of study in molecular biology and biomedical research.
