Analytical Data
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Gene name
GJB3
- Application
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Alternative Names
GJB3; CX31; Gap junction beta-3 protein; Connexin-31; Cx31
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O75712
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Expression Region
1-270aa
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AA Sequence
MDWKTLQALLSGVNKYSTAFGRIWLSVVFVFRVLVYVVAAERVWGDEQKDFDCNTKQPGCTNVCYDNYFPISNIRLWALQLIFVTCPSLLVILHVAYREERERRHRQKHGDQCAKLYDNAGKKHGGLWWTYLFSLIFKLIIEFLFLYLLHTLWHGFNMPRLVQCANVAPCPNIVDCYIARPTEKKIFTYFMVGASAVCIVLTICELCYLICHRVLRGLHKDKPRGGCSPSSSASRASTCRCHHKLVEAGEVDPDPGNNKLQASAPNLTPI
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Molecular Weight
55.44 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GJB3, also known as connexin 31, is a member of the connexin family of proteins that form gap junctions, allowing for direct intercellular communication. Mutations in the GJB3 gene are linked to various genetic disorders, primarily non-syndromic hearing loss (NSHL), particularly in certain populations. Understanding the structure and function of GJB3 is crucial for elucidating the molecular mechanisms underlying these diseases, as gap junctions play essential roles in maintaining cellular homeostasis and facilitating communication between adjacent cells. Recent advancements in recombinant protein technology have enabled researchers to produce and study GJB3 in vitro, providing insights into its functional properties and the impact of specific mutations on protein behavior. This research not only enhances our understanding of the pathophysiology associated with GJB3 mutations but also serves as a foundation for exploring potential therapeutic strategies for hearing loss and other related conditions. Investigating GJB3’s role in cellular pathways and its interactions with other proteins could lead to novel approaches in treating auditory and other connexin-related disorders. Overall, the study of GJB3 recombinant protein is pivotal in bridging the gap between genetic mutations and the phenotypic manifestations of associated diseases, paving the way for advancements in molecular medicine and targeted treatments.
